Abstract
Bovine tuberculosis (bTB) is a disease of cattle that represents a risk to public health and causes severe economic losses to the livestock industry. Recently, genetic studies, like genome-wide association studies (GWAS) have greatly improved the investigation of complex diseases identifying thousands of disease-associated genomic variants. Here, we present evidence of genetic variants associated with resistance to TB in Mexican dairy cattle using a case-control approach with a selective DNA pooling experimental design. A total of 154 QTLRs (quantitative trait loci regions) at 10% PFP (proportion of false positives), 42 at 5% PFP and 5 at 1% PFP have been identified, which harbored 172 annotated genes. On BTA13, five new QTLRs were identified in the MACROD2 and KIF16B genes, supporting their involvement in resistance to bTB. Six QTLRs harbor seven annotated genes that have been previously reported as involved in immune response against Mycobacterium spp: BTA (Bos taurus autosome) 1 (CD80), BTA3 (CTSS), BTA 3 (FCGR1A), BTA 23 (HFE), BTA 25 (IL21R), and BTA 29 (ANO9 and SIGIRR). We identified novel QTLRs harboring genes involved in Mycobacterium spp. immune response. This is a first screening for resistance to TB infection on Mexican dairy cattle based on a dense SNP (Single Nucleotide Polymorphism) chip.
Highlights
Bovine tuberculosis, caused by Mycobacterium bovis is a chronic infectious disease characterized by granulomas in affected tissues [1,2]
The aim of this study was to identify QTL regions involved in resistance to TB in Mexican dairy cattle using a genome-wide association studies (GWAS) case-control approach with a selective DNA pooling experimental design
The results presented here reveal novel QTL region (QTLR) and confirm mapped loci for resistance to tuberculosis in dairy cattle
Summary
Bovine tuberculosis (bTB), caused by Mycobacterium bovis is a chronic infectious disease characterized by granulomas in affected tissues [1,2]. M. bovis infects a wide range of mammalian hosts, domestic and wildlife species, and humans; it is a risk to public health [3]. It has been estimated that nearly 10 million people are affected by tuberculosis worldwide every year, and that the proportion of cases due to M. bovis in humans during the last two decades was from. BTB causes economic losses to the livestock industry: infected animals have poor production performance, die or are disposed of prematurely [7,8]. Cattle TB is considered the fourth most significant livestock disease in terms of impact on human health in developing countries, including risks to species other than cattle and the wildlife species [9]. The program relies on a test-and-slaughter strategy in herds of cattle, and carcass inspection at abattoirs [10]
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