Genome-Wide Association Mapping for Tolerance to Preharvest Sprouting and Low Falling Numbers in Wheat.

Shantel A Martinez,Camille M Steber,Meng Huang,Zhiwu Zhang,Arron H Carter,Jayfred Godoy,Kimberly A Garland Campbell

doi:10.3389/fpls.2018.00141

Shantel A Martinez, Camille M Steber + Show 5 more

Open Access

https://doi.org/10.3389/fpls.2018.00141

Copy DOI

Abstract

Preharvest sprouting (PHS), the germination of grain on the mother plant under cool and wet conditions, is a recurring problem for wheat farmers worldwide. α-amylase enzyme produced during PHS degrades starch resulting in baked good with poor end-use quality. The Hagberg-Perten Falling Number (FN) test is used to measure this problem in the wheat industry, and determines how much a farmer's wheat is discounted for PHS damage. PHS tolerance is associated with higher grain dormancy. Thus, breeding programs use germination-based assays such as the spike-wetting test to measure PHS susceptibility. Association mapping identified loci associated with PHS tolerance in U.S. Pacific Northwest germplasm based both on FN and on spike-wetting test data. The study was performed using a panel of 469 white winter wheat cultivars and elite breeding lines grown in six Washington state environments, and genotyped for 15,229 polymorphic markers using the 90k SNP Illumina iSelect array. Marker-trait associations were identified using the FarmCPU R package. Principal component analysis was directly and a kinship matrix was indirectly used to account for population structure. Nine loci were associated with FN and 34 loci associated with PHS based on sprouting scores. None of the QFN.wsu loci were detected in multiple environments, whereas six of the 34 QPHS.wsu loci were detected in two of the five environments. There was no overlap between the QTN detected based on FN and PHS, and there was little correlation between the two traits. However, both traits appear to be PHS-related since 19 of the 34 QPHS.wsu loci and four of the nine QFN.wsu loci co-localized with previously published dormancy and PHS QTL. Identification of these loci will lead to a better understanding of the genetic architecture of PHS and will help with the future development of genomic selection models.

Highlights

Rainy conditions before harvest can cause mature grain to initiate germination while still on the mother plant (Rodríguez et al, 2015)
A panel of 469 soft white wheat accessions was evaluated for Preharvest sprouting (PHS) tolerance based on spike-wetting tests and Falling Number (FN) following rain in the field
Based on the fact that the strong QPHS.wsu-2D locus was near the C locus region for compact spike morphology, we examined whether the club wheat breeding program contributed more PHS tolerance to the genome-wide association mapping study (GWAS) than the lax wheat breeding programs (Figure 3)

Summary

Introduction

Rainy conditions before harvest can cause mature grain to initiate germination while still on the mother plant (Rodríguez et al, 2015) This problem, called preharvest sprouting (PHS), occurs in many cereal crops such as wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), and sorghum (Sorghum bicolor) (Paterson and Sorrells, 1990; Gualano et al, 2007; Ullrich et al, 2009). The degree of wheat grain dormancy and PHS tolerance depends on environmental conditions both before and after the grain reaches physiological maturity. If rain and cold temperatures occur after physiological maturity, seed dormancy can be broken through cold stratification and grain is more likely to sprout. Since lack of red kernel color reduces dormancy, other genetic mechanisms supporting PHS tolerance must be identified and selected in wheat with white kernels

Methods

Results

Conclusion