Abstract
BackgroundPentatricopeptide-repeat proteins (PPRs) are characterized by tandem arrays of a degenerate 35-amino-acid (PPR motifs), which can bind RNA strands and participate in post-transcription. PPR proteins family is one of the largest families in land plants and play important roles in organelle RNA metabolism and plant development. However, the functions of PPR genes involved in biotic and abiotic stresses of rice (Oryza sativa L.) remain largely unknown.ResultsIn the present study, a comprehensive genome-wide analysis of PPR genes was performed. A total of 491 PPR genes were found in the rice genome, of which 246 PPR genes belong to the P subfamily, and 245 genes belong to the PLS subfamily. Gene structure analysis showed that most PPR genes lack intron. Chromosomal location analysis indicated that PPR genes were widely distributed in all 12 rice chromosomes. Phylogenetic relationship analysis revealed the distinct difference between the P and PLS subfamilies. Many PPR proteins are predicted to target chloroplasts or mitochondria, and a PPR protein (LOC_Os10g34310) was verified to localize in mitochondria. Furthermore, three PPR genes (LOC_Os03g17634,LOC_Os07g40820,LOC_Os04g51350) were verified as corresponding miRNA targets. The expression pattern analysis showed that many PPR genes could be induced under biotic and abiotic stresses. Finally, seven PPR genes were confirmed with their expression patterns under salinity or drought stress.ConclusionsWe found 491 PPR genes in the rice genome, and our genes structure analysis and syntenic analysis indicated that PPR genes might be derived from amplification by retro-transposition. The expression pattern present here suggested that PPR proteins have crucial roles in response to different abiotic stresses in rice. Taken together, our study provides a comprehensive analysis of the PPR gene family and will facilitate further studies on their roles in rice growth and development.
Highlights
Pentatricopeptide-repeat proteins (PPRs) are characterized by tandem arrays of a degenerate 35-aminoacid (PPR motifs), which can bind RNA strands and participate in post-transcription
Pentatricopeptide repeat (PPR) proteins are a group of proteins that contain tandem repeats of degenerate 35-amino-acid motifs (PPR motifs) [1, 2], which play an important role in plant growth and development
Identification of PPR genes in the rice genome A total of 491 PPR genes were identified in the rice genome in this study (Additional file 1: Table S1)
Summary
Pentatricopeptide-repeat proteins (PPRs) are characterized by tandem arrays of a degenerate 35-aminoacid (PPR motifs), which can bind RNA strands and participate in post-transcription. Pentatricopeptide repeat (PPR) proteins are a group of proteins that contain tandem repeats of degenerate 35-amino-acid motifs (PPR motifs) [1, 2], which play an important role in plant growth and development. Based on the P, L and S motifs, PPR proteins can be divided into two subfamilies, P and PLS [4]. P subfamily proteins contain tandem arrays of P motifs without other PPR motifs, while PLS subgroup proteins only contain varied PPR motifs, sometimes with a non-PPR motif, DYW
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
