Genome-wide analysis of MYB family genes in Tripterygium wilfordii and their potential roles in terpenoid biosynthesis.

Abstract

Terpenoids are a class of significant bioactive components in the woody vine of Tripterygium wilfordii. Previous studies have shown that MYB transcription factors play important roles in plant secondary metabolism, growth, and developmental processes. However, the MYB involved in terpenoid biosynthesis in Tripterygium wilfordii are unknown. To identify Tripterygium wilfordii MYB (TwMYB) genes that are involved in terpenoid biosynthesis, we conducted the genome‐wide analysis of the TwMYB gene family. A total of 207 TwMYBs were identified including 84 1R‐TwMYB, 117 R2R3‐TwMYB, four 3R‐TwMYB, and two 4R‐TwMYB genes. The most abundant R2R3‐TwMYBs together with their Arabidopsis homologs were categorized into 26 subgroups. Intraspecific collinearity analysis found that the 74.9% of the TwMYBs may be generated by segmental duplication events, and 36.7% of duplicated gene pairs were derived from the specific whole genome duplication (WGD) event in Tripterygium wilfordii. In addition, interspecies collinearity analysis found that 16 TwMYB genes formed homologous gene pairs with MYB genes in seven representative species, which indicated they may have a key role in evolution. Notably, we found that the TwMYB genes were differentially expressed in various tissues by expression pattern analysis. In order to further select the candidate genes related to terpenoid biosynthesis, the assay of Methyl jasmonate (MeJA) induction and analysis of phylogenetic tree was conducted. It was speculated that six candidate TwMYB genes (TwMYB33, TwMYB34, TwMYB45, TwMYB67, TwMYB102, and TwMYB103) are involved in regulating terpenoid biosynthesis. This study is the first systematic analysis of the TwMYB gene family and will lay a foundation for the functional characterization of TwMYB genes in the regulation of terpenoid biosynthesis.