Genome-wide analysis of lectin receptor-like kinases family from potato (Solanum tuberosum L.).

Weina Zhang,Mingfu Shi,Shuhao Qin,Xinyu Yang,Yichen Kang,Yuhui Liu,Kai Yao,Yanling Fan,Zhongjian Chen

doi:10.7717/peerj.9310

Weina Zhang, Mingfu Shi + Show 7 more

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https://doi.org/10.7717/peerj.9310

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Abstract

Lectin receptor-like kinases (LecRLKs) are involved in responses to diverse environmental stresses and pathogenic microbes. A comprehensive acknowledgment of the family members in potato (Solanum tuberosum) genome is largely limited until now. In total, 113 potato LecRLKs (StLecRLKs) were first identified, including 85 G-type, 26 L-type and 2 C-type members. Based on phylogenetic analysis, StLecRLKs were sub-grouped into seven clades, including C-type, L-type, G-I, G-II, G-III G-IV and G-V. Chromosomal distribution and gene duplication analysis revealed the expansion of StLecRLKs occurred majorly through tandem duplication although the whole-genome duplication (WGD)/segmental duplication events were found. Cis-elements in the StLecRLKs promoter region responded mainly to signals of defense and stress, phytohormone, biotic or abiotic stress. Moreover, expressional investigations indicated that the family members of the clades L-type, G-I, G-IV and G-V were responsive to both bacterial and fungal infection. Based on qRT-PCR analysis, the expressions of PGSC0003DMP400055136 and PGSC0003DMP400067047 were strongly induced in all treatments by both Fusarium sulphureum (Fs) and Phytophthora infestans (Pi) inoculation. The present study provides valuable information for LecRLKs gene family in potato genome, and establishes a foundation for further research into the functional analysis.

Highlights

Unlike animals, plants lack the basic survival ability to escape from the danger of environmental fluctuations
Lectin receptor-like kinases (LecRLKs) annotation and classification Based on domain screening, a total of 113 transcripts encoding LecRLKs were identified in potato, including 85 G-type, 26 L-type and 2 C-type members (File S2)
80, 76, 139 and 167 LecRLKs were annotated in Tomato (Solanum lycopersicum), Arabidopsis, Rice (Oryza sativa) and Apple (Malus domestica), respectively

Summary

Introduction

Plants lack the basic survival ability to escape from the danger of environmental fluctuations. Receptor-like kinases (RLKs), as one of the largest receptors, allow the plants to communicate between cells and to interact with the environment (Li et al, 2018). RLKs have an important role in response to biotic and abiotic stresses (Nazarian-Firouzabadi et al, 2019). Typical RLKs comprise an extracellular ligand binding domain in N terminus and an intercellular cytoplasmic kinase domain in C terminus, and the two regions are connected via a transmembrane domain. How to cite this article Zhang W, Chen Z, Kang Y, Fan Y, Liu Y, Yang X, Shi M, Yao K, Qin S. Genome-wide analysis of lectin receptor-like kinases family from potato (Solanum tuberosum L.).

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