Abstract
BBX (B-box), a zinc finger transcription factor with one or two B-box domains, plays an important role in plant photomorphogenesis, growth, and development as well as response to environmental changes. In this study, 28 Tartary buckwheat BBX (FtBBX) genes were identified and screened using a comparison program. Their physicochemical properties, gene structures, conserved motifs, distribution in chromosomal, and phylogeny of the coding proteins, as well as their expression patterns, were analyzed. In addition, multiple collinearity analysis in three monocots and three dicot species illustrated that the BBX proteins identified from monocots clustered separately from those of dicots. Moreover, the expression of 11 candidate BBX genes with probable involvement in the regulation of anthocyanin biosynthesis was analyzed in the sprouts of Tartary buckwheat during light treatment. The results of gene structure analysis showed that all the 28 BBX genes contained B-box domain, three genes lacked introns, and these genes were unevenly distributed on the other seven chromosomes except for chromosome 6. The 28 proteins contained 10 conserved motifs and could be divided into five subfamilies. BBX genes of Tartary buckwheat showed varying expression under different conditions demonstrating that FtBBXs might play important roles in Tartary buckwheat growth and development. This study lays a foundation for further understanding of Tartary buckwheat BBX genes and their functions in growth and development as well as regulation of pigmentation in Tartary buckwheat.
Highlights
Zinc finger protein (ZFP) transcription factors are a large class of transcription factors, capable of interacting with DNA, RNA and proteins, and regulate transcription, RNA packaging, apoptosis, protein folding and assembly, among others (Noman et al, 2019)
After the repeat sequences were removed from the preliminary candidate sequences, the conserved domains were analyzed by the Conserved Domain Database (CDD) database, and the protein sequences without BBX domain were deleted, and the members of Tartary buckwheat BBX transcription factor family were obtained
Twenty-eight BBX family sequences were identified in Tartary buckwheat, which is similar with the number of BBX genes in Arabidopsis (32) and rice (30)
Summary
Zinc finger protein (ZFP) transcription factors are a large class of transcription factors, capable of interacting with DNA, RNA and proteins, and regulate transcription, RNA packaging, apoptosis, protein folding and assembly, among others (Noman et al, 2019). Based on the protein structure, ZFP can be divided into several subfamilies. BBX (B-box) is a subfamily of zinc finger structural protein family that occurs in all eukaryotes from single-celled to multicellular organisms (Crocco & Botto, 2013; Fang et al, 2019).
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