Abstract
MicroRNA-mediated post-transcriptional regulation has been reported on ROS production and scavenging systems. Although microRNAs first appeared highly conserved among plant species, several aspects of biogenesis, function and evolution of microRNAs were shown to differ. High throughput transcriptome and degradome analyses enable to identify small RNAs and their mRNA targets. A non-photosynthetic tissue particularly prone to redox reactions, laticifers from Hevea brasiliensis, revealed species-specific post-transcriptional regulations. This paper sets out to identify the 407 genes of the thirty main redox-related gene families harboured by the Hevea genome. There are 161 redox-related genes expressed in latex. Thirteen of these redox-related genes were targeted by 11 microRNAs. To our knowledge, this is the first report on a mutation in the miR398 binding site of the cytosolic CuZnSOD. A working model was proposed for transcriptional and post-transcriptional regulation with respect to the predicted subcellular localization of deduced proteins.
Highlights
MicroRNA-mediated post-transcriptional regulation has been reported on Reactive oxygen species (ROS) production and scavenging systems
Of the 161 redox-related genes expressed in latex, 27 genes were shown to be targeted by microRNAs using small RNAs and degradome analyses
Hevea has a much larger number of redox-related genes (407) compared to Arabidopsis (306). This is mainly explained by the absence of genes encoding polyphenol oxidase in Arabidopsis when Hevea genome harboured genes, and by a smaller number of genes encoding glutaredoxin (43), glutathione S-transferase (51) and peroxidase (73) in Arabidopsis compared to Hevea (51, 77 and 114, respectively)
Summary
High levels of ROS such as 1O2 (singlet oxygen), O2°− (superoxide radical), °OH (hydroxyl radical) and H2O2 (hydrogen peroxide) are generated during abiotic and biotic stress, as well as some plant development processes This oxidative stress triggers disturbances in the basal redox state[1]. MicroRNA-mediated post-transcriptional regulation has been reported on ROS production and scavenging systems This control can occur by transcript cleavage of either redox-related genes[4,5], or their upstream transcription factors[6], as well as indirectly through the repression of genes that induce hormone changes[7] or a response to stress[8]. When ROS-scavenging systems cannot offset ROS accumulation, cellular dysfunctions lead to the agglutination of rubber particles[19,20] This physiological syndrome, called Tapping Panel Dryness (TPD), is responsible for major losses in natural rubber production[21]
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