Abstract
Titanium dioxide nanotube arrays (TNAs) provide a promising platform for medical implants and nanomedicine applications. The present cell–TNA study has provided profound understanding on protection of genome integrity via telomere, telomerase and NF-κB activities using an epithelial cell model. It has been revealed in this study that cell–TNA interaction triggers the telomere shortening activity and inhibition of telomerase activity at the mRNA and protein level. The present work supported that the cell–TNA stimulus might involve controlled transcription and proliferative activities via NBN and TERF21P mechanisms. Moreover, inhibition of NF-κB may promote molecular sensitivity via senescence-associated secretory phenotype activities and might result in reduced inflammatory response which would be good for cell and nanosurface adaptation activities. Thus, this nanomaterial-molecular knowledge is beneficial for further nanomaterial characterization and advanced medical application.
Highlights
Titanium dioxide nanotube arrays (TNAs) have been widely studied as a nanosurface for biomedical applications especially in biosensors, drug delivery, diagnostics, and dental and orthopedic implants.[1]
The analysis indicated the presence of decreased telomeric signals in cells cultivated on TNA surface (20% positive cells) in comparison with reference (40% positive cells)
terminal restriction fragment (TRF) value of cells grown on TNA (3.0 Kbp) was lower compared to cells grown on control surfaces such as P (4.2 Kbp), G (4.0 Kbp) and TiP (3.5 Kbp)
Summary
Titanium dioxide nanotube arrays (TNAs) have been widely studied as a nanosurface for biomedical applications especially in biosensors, drug delivery, diagnostics, and dental and orthopedic implants.[1] The nanometric scaled topography of biomedical products plays a decisive role in the surrounding tissue acceptance, cellular stability and cell survival.[2] The fate of any cell is determined by its adaptive capacity to an environment.[3] The mislaying of decisions related to the cell's fate may result in cellular transformation or carcinogenesis risk.[4]. Studying the nanomaterial potential interaction at the molecular level of the cells including proteins and DNA may allow further understanding on nanomaterial and genome integrity. Bio-indicators for genome integrity such as telomere and telomerase crucial for both cancer and aging via cellular
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