Abstract
Previous studies have shown multiple mechanisms and pathophysiological changes after anesthesia, and genome-wide studies have been implemented in the studies of brain aging and neurodegenerative diseases. However, the genome-wide gene expression patterns and modulation networks after general anesthesia remains to be elucidated. Therefore, whole transcriptome microarray analysis was used to explore the coding gene expression patterns in the hippocampus of aged rats after sevoflurane anesthesia. Six hundred and thirty one upregulated and 183 downregulated genes were screened out, then 44 enriched terms of biological process, 16 of molecular function and 18 of the cellular components were identified by Gene Ontology (GO) and KEGG analysis. Among them, oxidative stress, metabolism, aging, and neurodegeneration were the most enriched biological processes and changed functions. Thus, involved genes of these processes were selected for qPCR verification and a good consistency was confirmed. The potential signaling pathways were further constructed including mitochondrion and oxidative stress-related Hifs-Prkcd-Akt-Nfe2l2-Sod1 signaling, multiple metabolism signaling (Scd2, Scap-Hmgcs2, Aldh18a1-Glul and Igf1r), as well as aging and neurodegeneration related signaling (Spidr-Ercc4-Cdkn1a-Pmaip1 and Map1lc3b). These results provide potential therapeutic gene targets for brain function modulation and memory formation process after inhaled anesthesia in the elderly, which could be valuable for preventing postoperative brain disorders and diseases, such as perioperative neurocognitive disorders (PND), from the genetic level in the future.
Highlights
Neurodegenerations, including Alzheimer’s disease (AD), represent important causes for the brain’s aging processes and related cognitive dysfunction and dementia (Wyss-Coray, 2016)
The genome-wide map of all autosomal and heterosomal coding and complex genes was represented as a circular ideogram, composed of concentric circles depicting the entire autosome complement, with chromosomal location annotated in a clockwise manner and statistical significance indicated by radial arrangements and color codes
The results of DAVID for Gene Ontology (GO) and KEGG analysis revealed that 44 terms of biological process, 16 terms of molecular function, and 18 terms of the cellular component were significantly enriched after sevoflurane anesthesia (p < 0.05), respectively
Summary
Neurodegenerations, including Alzheimer’s disease (AD), represent important causes for the brain’s aging processes and related cognitive dysfunction and dementia (Wyss-Coray, 2016). Genome-wide association studies, using microarray platforms, have allowed a deeper understanding of the molecular factors involved in the pathophysiology of degenerated brain disease. These studies identified multiple susceptibility loci for neurodegeneration (Harold et al, 2009; Lambert et al, 2009; Seshadri et al, 2010; Hollingworth et al, 2011), and these genes were clustered into pathways including inflammation and immune response, lipid metabolism, endocytosis/intracellular trafficking (Kunkle et al, 2019). The are few studies about the gene network and pathophysiology for brain function modulation during the perioperative context
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
