Abstract
BackgroundSweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. β-galactosidase (bgal) is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation. However, the function of bgal genes in sweetpotato remains unclear.ResultsIn this study, 17 β-galactosidase genes (Ibbgal) were identified in sweetpotato, which were classified into seven subfamilies using interspecific phylogenetic and comparative analysis. The promoter regions of Ibbgals harbored several stress, hormone and light responsive cis-acting elements. Quantitative real-time PCR results displayed that Ibbgal genes had the distinct expression patterns across different tissues and varieties. Moreover, the expression profiles under various hormonal treatments, abiotic and biotic stresses were highly divergent in leaves and root.ConclusionsTaken together, these findings suggested that Ibbgals might play an important role in plant development and stress responses, which provided evidences for further study of bgal function and sweetpotato breeding.
Highlights
Sweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. βgalactosidase is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation
Identification and characterization of Ibbgal genes in sweetpotato A total of 17 Ibbgal genes were isolated from sweetpotato after local Basic local alignment search tool (BLAST) using the conserved bgal domain
Characteristic analysis showed that these 17 Ibbgals were 673 to 1110 aa in length, the predicted molecular weights (MW) and pIs ranged from 74.8 kDa to 125.1 kDa and 5.31 to 6.16, respectively
Summary
Identification and characterization of Ibbgal genes in sweetpotato A total of 17 Ibbgal genes were isolated from sweetpotato after local BLAST using the conserved bgal domain. The expression of all Ibbgal was greatly up-regulated by salt and ABA treatments in the leaves of sweetpotato Taken together, these Ibbgal genes play essential functions in response to biotic and abiotic stresses and their related signal transduction pathways. Further studies need to be performed to investigate the functions of bgals on the stress-response system in sweetpotato
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