Abstract
Plant 14-3-3 proteins act as critical components of various cellular signaling processes and play an important role in regulating multiple physiological processes. However, less information is known about the 14-3-3 gene family in banana. In this study, 25 14-3-3 genes were identified from the banana genome. Based on the evolutionary analysis, banana 14-3-3 proteins were clustered into ε and non-ε groups. Conserved motif analysis showed that all identified banana 14-3-3 genes had the typical 14-3-3 motif. The gene structure of banana 14-3-3 genes showed distinct class-specific divergence between the ε group and the non-ε group. Most banana 14-3-3 genes showed strong transcript accumulation changes during fruit development and postharvest ripening in two banana varieties, indicating that they might be involved in regulating fruit development and ripening. Moreover, some 14-3-3 genes also showed great changes after osmotic, cold, and salt treatments in two banana varieties, suggested their potential role in regulating banana response to abiotic stress. Taken together, this systemic analysis reveals the involvement of banana 14-3-3 genes in fruit development, postharvest ripening, and response to abiotic stress and provides useful information for understanding the functions of 14-3-3 genes in banana.
Highlights
As a large annual monocotyledonous herbaceous plant, banana (Musa acuminata L.) is one of the most popular fresh fruit
A total of 25 non-redundant 14-33 genes were identified in the banana genome, which was supported by conserved domain and multiple sequence alignment analyses (Figure 1, Table S1)
The 25 predicated banana 14-3-3 proteins ranged from 130 (MaGRF23, MaGRF24) to 344 (MaGRF2) amino acid residues in length, and their relative molecular mass varied from 14.529 KDa (MaGRF24) to 38.789 KDa (MaGRF2), with the pIs in the range of 4.31–6.41, suggesting their potentially different roles in regulating cellular processes under different environments (Figure 1, Table S2)
Summary
As a large annual monocotyledonous herbaceous plant, banana (Musa acuminata L.) is one of the most popular fresh fruit. Banana fruit quality is determined by development and postharvest ripening processes and it plays an important role in the commodity economy (Raza et al, 2016). It is necessary to study the molecular mechanism underlying banana fruit development, postharvest ripening, and response to various abiotic stresses. Considering the importance of 14-3-3 proteins in regulating plant growth, development, as well as responses to abiotic stresses, a comprehensive analysis of banana 14-3-3 genes was conducted. A total of 25 14-3-3s genes were identified in banana. The expression of banana 14-3-3 genes in various organs, different phases of fruit development and ripening, and responses to various stresses in Ba Xi Jiao The detailed characterization of the banana 14-3-3 gene family provides a foundation and useful genetic resources for further functional characterization of potential targets of 14-3-3 and the genetic improvement of bananas
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