Genome-wide identification of wheat (Triticum aestivum) expansins and expansin expression analysis in cold-tolerant and cold-sensitive wheat cultivars.

Jun-Feng Zhang,Feng-Lan Li,Yu Miao,Xu Wang,Shan-Shan Feng,Jia-Min Dong,Yong-Qing Xu,Xu Feng,Li-Na Peng,Qiao-Qin Zhao,Shu-Kuan Yao,Bao-Zhong Hu,Fei Li,Genlou Sun

doi:10.1371/journal.pone.0195138

Jun-Feng Zhang, Feng-Lan Li + Show 12 more

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https://doi.org/10.1371/journal.pone.0195138

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Journal: PloS one	Publication Date: Mar 29, 2018
Citations: 23	License type: CC BY 4.0

Affiliation: Northeast Agricultural University, Harbin University

Abstract

Plant expansins are proteins involved in cell wall loosening, plant growth, and development, as well as in response to plant diseases and other stresses. In this study, we identified 128 expansin coding sequences from the wheat (Triticum aestivum) genome. These sequences belong to 45 homoeologous copies of TaEXPs, including 26 TaEXPAs, 15 TaEXPBs and four TaEXLAs. No TaEXLB was identified. Gene expression and sub-expression profiles revealed that most of the TaEXPs were expressed either only in root tissues or in multiple organs. Real-time qPCR analysis showed that many TaEXPs were differentially expressed in four different tissues of the two wheat cultivars—the cold-sensitive ‘Chinese Spring (CS)’ and the cold-tolerant ‘Dongnongdongmai 1 (D1)’ cultivars. Our results suggest that the differential expression of TaEXPs could be related to low-temperature tolerance or sensitivity of different wheat cultivars. Our study expands our knowledge on wheat expansins and sheds new light on the functions of expansins in plant development and stress response.

Highlights

Plant expansins are a family of non-enzymatic proteins present in the plant cell wall
TaEXPA1-11, TaEXPB1-11 and TaEXPB23 had been named previously [28, 52,53,54], so the newly identified expansins were named as TaEXPA12 to –29, TaEXPB12 to –24 except for two that were named TaEXPB23 and TaEXLA1 to –4 (Table 1)
Expansins are a large family of proteins that play broad roles in plant development, from the emergence of root hairs to fruit softening

Summary

Introduction

Plant expansins are a family of non-enzymatic proteins present in the plant cell wall They play important roles in cell wall modification, which is critical for cell growth and other biological processes [1]. Expansins are involved in leaf initiation and expansion [11], flowering and determining flower size [12], pollen germination and fertilization [13], and fruit growth and/or ripening [14, 15]. They were associated with nutrient-uptake efficiency [16] as well as with abiotic and biotic stress tolerance [4, 17].

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