Abstract
Two-component system (TCS) is responsible for cytokinin signaling, which plays critical roles in plant development and physiological process. This system is generally composed of two signaling factors, a histidine kinase (HK) and a response regulator (RR) that is associated with a histidine phosphotransfer (HP) protein. In this study, we performed systematic investigation on TCS genes in melon (Cucumis melon L.). We identified 44 TCS genes in melon, including 18 HK(L)s (9 HKs and 9 HKLs), 5 HPs (4 authentic and 1 pseudo), and 21 RRs (7 Type-A, 8 Type-B, and 6 pseudo). The classification and structure of these melon TCS members were introduced in detail as well. Our results provided new insights into the characteristics of the melon TCS genes and might benefit their functional study in future.
Highlights
Cytokinins are essential for many of the physiological and developmental processes such as seed germination, functional root nodule establishment, lateral root development, shoot apical meristem maintenance, leaf expansion, flowering, circadian clock, nutrient mobilization, abiotic stress, and senescence [1] [2][3] [4]
Protein sequence data of Arabidopsis Arabidopsis histidine-kinase (AHK), Arabidopsis histidine phosphotransfer (AHP) and Arabidopsis response regulator (ARR) were downloaded from the NCBI databases
two-component system (TCS) genes including 26 histidine kinase (HK)(L)s, 6 histidine phosphotransfer (HP), 58 RRs in the melon genome. To confirm these putative melon TCS genes, the amino acid sequences of all 90 genes were further filtered by Pfam and SMART based on the presence of structural and conserved TCS elements
Summary
Cytokinins are essential for many of the physiological and developmental processes such as seed germination, functional root nodule establishment, lateral root development, shoot apical meristem maintenance, leaf expansion, flowering, circadian clock, nutrient mobilization, abiotic stress, and senescence [1] [2]. HK can sense the cytokinin signals by phosphorylating its conserved histidine residues. Melon (Cucumis melon L.) is an economically important fruit crop that originates from Asian, with an average production during the past decade more than 29 million tons per year (FAOSTAT, 2017; http://www.fao.org/faostat/en/#home). This crop is mainly cultivated in tropical and temperate countries, especially in the Asian countries, with China leading the list [10] [11]. We examined the putative TCS genes and revealed that the melon genome contained a total of 44 members. Their classification and characteristics were analyzed systematically. Our comprehensive analysis of the TCS genes might provide a framework for future functional dissection of TCSs in melon hormone signal transduction
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
