Genome-wide identification of trihelix transcription factors in the apple genome in silico.

Abstract

Trihelix transcription factors are involved in the growth and development of plants, as well as various stress responses. In the study presented, we identified 37 trihelix family genes in the apple genome (MdTH). The trihelix genes were located on 13 chromosomes. Phylogenetic analysis of these MdTH and the trihelix genes of other species divided them into six subfamilies: GT-1, GT-2, SH4, SIP1, GTγ, and GTδ. The genes of different groups significantly diverged in their gene structure and conserved functional domains. Cis-element analysis showed that promoter sequences of MdTH genes contained light response elements, phytohormone response elements, and stress-related cis-elements. The expression pattern analysis results demonstrated that MdTH were regulated by drought, salinity, as well as high and low temperatures. MdTH4 and MdTH24 were highly regulated by soil salinity, MdTH4-by drought. MdTH30 showed high expression under low temperature; MdTH8, MdTH20, and MdTH36-under high temperature.