Abstract
MYB transcription factors of plants play important roles in flavonoid synthesis, aroma regulation, floral organ morphogenesis, and responses to biotic and abiotic stresses. Cymbidium ensifolium is a perennial herbaceous plant belonging to Orchidaceae, with special flower colors and high ornamental value. In this study, a total of 136 CeMYB transcription factors were identified from the genome of C. ensifolium, including 27 1R-MYBs, 102 R2R3-MYBs, 2 3R-MYBs, 2 4R-MYBs, and 3 atypical MYBs. Through phylogenetic analysis in combination with MYB in Arabidopsis thaliana, 20 clusters were obtained, indicating that these CeMYBs may have a variety of biological functions. The 136 CeMYBs were distributed on 18 chromosomes, and the conserved domain analysis showed that they harbored typical amino acid sequence repeats. The motif prediction revealed that multiple conserved elements were mostly located in the N-terminal of CeMYBs, suggesting their functions to be relatively conserved. CeMYBs harbored introns ranging from 0 to 13 and contained a large number of stress- and hormone-responsive cis-acting elements in the promoter regions. The subcellular localization prediction demonstrated that most of CeMYBs were positioned in the nucleus. The analysis of the CeMYBs expression based on transcriptome data showed that CeMYB52, and CeMYB104 of the S6 subfamily may be the key genes leading to flower color variation. The results lay a foundation for the study of MYB transcription factors of C. ensifolium and provide valuable information for further investigations of the potential function of MYB genes in the process of anthocyanin biosynthesis.
Highlights
MYB proteins constitute one of the largest transcription factor families in plants [1].The members of the MYB family have a conserved DNA-binding domain at the Nterminus, which is usually composed of up to four imperfect amino acid repeats harboring50–53 amino acids [2,3]
Subgroup 4 (S4), subgroup (S5), subgroup (S6), and subgroup (S7) of the MYB in Arabidopsis thaliana are highly correlated with the flavonoid biosynthetic pathway, among which S4 is related to the phenylpropane metabolic pathway [9], S5 plays an important role in the proanthocyanidin metabolic pathway, S6 is involved in the anthocyanin biosynthetic pathway (ABP), and S7 participates in the flavonol synthetic pathway [10]
A total of 140 CeMYBs transcription factors were initially identified by BLAST searches using whole Arabidopsis MYB protein sequences as the query, and they were screened out by Pfam
Summary
MYB proteins constitute one of the largest transcription factor families in plants [1].The members of the MYB family have a conserved DNA-binding domain at the Nterminus, which is usually composed of up to four imperfect amino acid repeats harboring50–53 amino acids [2,3]. MYB proteins constitute one of the largest transcription factor families in plants [1]. The members of the MYB family have a conserved DNA-binding domain at the Nterminus, which is usually composed of up to four imperfect amino acid repeats harboring. Numerous studies have shown that R2R3-MYB transcription factors are involved in flavonoids biosynthesis. Subgroup 4 (S4), subgroup (S5), subgroup (S6), and subgroup (S7) of the MYB in Arabidopsis thaliana are highly correlated with the flavonoid biosynthetic pathway, among which S4 is related to the phenylpropane metabolic pathway [9], S5 plays an important role in the proanthocyanidin metabolic pathway, S6 is involved in the anthocyanin biosynthetic pathway (ABP), and S7 participates in the flavonol synthetic pathway [10]. MBW protein complex, a combination of MYB, bHLH transcription factors, and WD40 proteins, play an important role in regulating the transcription of structural genes [13]
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