Genome-wide identification of the CLAVATA3/EMBRYO SURROUNDING REGION (CLE) family in grape (Vitis vinifera L.)

Pengfei Wang,Yongmei Wang,Fengshan Ren

doi:10.1186/s12864-019-5944-2

Pengfei Wang, Yongmei Wang + Show 1 more

Open Access

https://doi.org/10.1186/s12864-019-5944-2

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Abstract

BackgroundCLE genes play various biological roles in plant growth and development, as well as in responses to environmental stimuli.ResultsIn the present study, we identified nine CLE genes in the grape genome using an effective identification method. We analyzed the expression profiles of grape CLE genes in different tissues and under environmental different stimuli. VvCLE3 was expressed in shoot apical meristem (SAM) enriched regions, and VvCLE6 was expressed in shoot tissue without SAM. When grapes were infected with bois noir, VvCLE2 was up-regulated. Under ABA treatment, VvCLE3 was down-regulated. VvCLE6 was up-regulated under high temperature stress. We found that VvCLE6 and VvCLE1 were highly expressed in root tissue. In addition, we compared the characteristics of CLEs from grape and other plant species. The CLE family in Sphagnum fallax underwent positive selection, while the CLE family in grape underwent purifying selection. The frequency of optimal codons and codon adaptation index of rice and grape CLE family members were positively correlated with GC content at the third site of synonymous codons, indicating that the dominant evolutionary pressure acting on rice and grape CLE genes was mutation pressure. We also found that closely related species had higher levels of similarity in relative synonymous codon usage in CLE genes. The rice CLE family was biased toward C and G nucleotides at third codon positions. Gene duplication and loss events were also found in grape CLE genes.ConclusionThese results demonstrate an effective identification method for CLE motifs and increase the understanding of grape CLEs. Future research on CLE genes may have applications for grape breeding and cultivation to better understand root and nodulation development.

Highlights

CLAVATA3/EMBRYO SURROUNDING REGION (CLE) genes play various biological roles in plant growth and development, as well as in responses to environmental stimuli
To clearly identify the CLE motif, we considered the above factors in a typical CLE motif
The following conserved motif parameters were used: motif length of 12–14 aa and output of two motifs, because past studies have shown that CLEs contain two CLE motifs [38]

Summary

Introduction

CLE genes play various biological roles in plant growth and development, as well as in responses to environmental stimuli. SSP signals relay information that coordinate cell proliferation and differentiation during plant development, and SSPs often bind to corresponding families of receptors and are CLEs contain a conserved 14-amino acid (aa) consensus sequence (conserved sequence motif KRXVPXGPNPLHNR), which is called the CLE motif or CLE domain [6]. Based on previous research that identified 12 amino acids as the conserved motif, the typical CLE motif has histidine (H1) or arginine (R1) at the first amino acid position. The research that indicated that the conserved motif is 13–14 amino acids long identified that the typical CLE motif has histidine (H2) or arginine (R2) at the second amino acid position [6]. The putative candidate CLEs in C. reinhardtii contained a potential conserved CLE motif, ALVPSGPERRHH, but it lacks the typical histidine (H1) or arginine (R1) at the first amino acid position [18]. Ohyama et al reported that CLV3 is a 13-amino-acid arabinosylated glycopeptide [14]

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