Abstract
棉花油脂合成相关代谢在控制油分形成、纤维发育等进程中起着重要作用。溶血磷脂酰基转移酶(LPAT)是植物油脂代谢过程中的一个关键酶。本研究利用雷蒙德氏棉全基因组数据库,通过生物信息学技术,鉴定并获得8个棉花LPAT家族基因的全序列和染色体定位等信息。通过序列比对进行系统进化和分类分析,明确这8个家族基因分布在6条染色体上,分为4亚类,其中第I类和第III类中各含2个基因、第II类1个、第IV类3个。组织表达分析表明这8个基因在棉花营养和生殖阶段均表现表达多样性。LPAT6和LPAT8在17 d胚珠中表达水平很高,推测在油脂合成代谢调控中起重要作用。8个LPAT基因均在纤维中优势表达,其中LPAT2、LPAT3和LPAT4表达水平更高,推测LPAT家族基因的表达参与棉纤维发育进程。
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