Genome-Wide Identification of Hsp90 Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses.

Charlotte Appiah,Zhong-Fu Yang,Jie He,Yang Wang,Gang Nie,Yong-Qun Zhu,Wen-Zhi Xu,Jie Zhou

doi:10.3390/plants10112509

Charlotte Appiah, Zhong-Fu Yang + Show 6 more

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https://doi.org/10.3390/plants10112509

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Abstract

The heat shock protein 90 (Hsp90) is a protein produced in plants in response to stress. This study identified and analyzed Hsp90 gene family members in the perennial ryegrass genome. From the results, eight Hsp90 proteins were obtained and their MW, pI and number of amino acid bases varied. The amino acid bases ranged from 526 to 862. The CDS also ranged from 20 (LpHsp0-4) to 1 (LpHsp90-5). The least number of CDS regions was 1 (LpHsp90-5) with 528 kb amino acids, while the highest was 20 (LpHsp90-4) with 862 kb amino acids, which showed diversity among the protein sequences. The phylogenetic tree revealed that Hsp90 genes in Lolium perenne, Arabidopsis thaliana, Oryza sativa and Brachypodium distachyon could be divided into two groups with five paralogous gene pairs and three orthologous gene pairs. The expression analysis after perennial ryegrass was subjected to heat, salt, chromium (Cr), cadmium (Cd), polyethylene glycol (PEG) and abscisic acid (ABA) revealed that LpHsp90 genes were generally highly expressed under heat stress, but only two LpHsp90 proteins were expressed under Cr stresses. Additionally, the expression of the LpHsp90 proteins differed at each time point in all treatments. This study provides the basis for an understanding of the functions of LpHsp90 proteins in abiotic stress studies and in plant breeding.

Highlights

Improving stress tolerance is among the major efforts of breeding advancement in cool season grass species [1]
Eight LpHsp90 genes were identified after the removal of redundant sequences from the genome database of perennial ryegrass
The analysis of the cis-acting elements of perennial ryegrass heat shock protein 90 (Hsp90) genes showed that plant hormone responsiveness was identified, implying that LpHsp90 genes might be involved in various plant stress-responsive pathways and closely related to the function of plant hormones such as abscisic acid, gibberellin and methyl-jasmonate (Table S1)

Summary

Introduction

Improving stress tolerance is among the major efforts of breeding advancement in cool season grass species [1]. Perennial ryegrass (Lolium perenne) is one of the major species of forage and turf grasses extensively planted in warm temperate to subtropical regions around the world, because it is to plant, has better tolerance to abiotic stresses, and requires low maintenance [2]. Achieving the potential yield after cultivation of perennial ryegrass is limited due to exposure to abiotic stresses in cultivated lands [3]. Abiotic stresses such as cold, drought, salinity, freezing, high light intensity and heat cause cell injury resulting in secondary stresses such as osmotic and oxidative stresses that critically impact the quality and yield of the perennial ryegrass plant [4,5,6]. The response of plants to heat shock is similar to that of other organisms when exposed to adverse stress conditions, producing highly conserved stress proteins called heat shock proteins (Hsp) [11,12,13]

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