Abstract
Peroxiredoxin (PRX) is a ubiquitous thioredoxin-dependent peroxidase that can eliminate excessive free radicals produced by stress and protect cells from oxidative damage. PRXs are also involved in reactive oxygen species (ROS)- and redox-dependent signaling by performing redox interactions with other proteins and modify their redox status. At present, PRX family identification, evolution and regulation research has been conducted in some plants; however, systematic research about this family is lacking in cotton. In this study, a total of 44 PRXs were identified in the cotton genome. Phylogenetic and conserved active site analyses showed that the PRXs were divided into six subfamilies according to the conserved site (PxxxTxxC…S…W/F) and conserved cysteinyl residues positions. Segmental duplication and polyploid events were the main methods for PRX family expansion, and the PRXs of diploid G. arboreum were the donors of PRXs in the D subgenomes of allotetraploid G. hirsutum and G. barbadense during the evolution of the PRX family. qRT-PCR analysis confirmed that cis-acting elements play important roles in regulating the expression of PRXs. Alternative splicing events occurred in GhPRX14-D that can increased the complexity of transcripts in G. hirsutum. Subcellular localization showed that most PRX members were located in chloroplasts, the cytoplasmic membrane and the nucleus. Our results provide systematic support for a better understanding of PRXs in cotton and a starting point for further studies of the specific functions of PRXs in cotton.
Highlights
The peroxiredoxin (PRX ) protein (EC 1.11.1.15) is a member of the thioredoxin-scaffold enzyme family and presents cysteine-dependent peroxidase activity against hydrogen peroxide substrates
G. raimondii (Gossypium raimondii L.,JGI) (Accession: PRJNA171262) (Paterson et al, 2012) and G. hirsutum (Gossypium hirsutum L., NAU) (Accession: PRJNA248163) (Zhang et al, 2015) genomic data files were obtained from the JGI database; G. arboreum (Gossypium arboreum L., CRI) (Accession: PRJNA382310) (Du et al, 2018) and G. barbadense (Gossypium barbadense L., Hau) (Accession: PRJNA433615) (Wang et al, 2018) genomic data files were downloaded from the CottonGen database
The protein databases of four cotton species (G. raimondii, G. arboreum, G. hirsutum, and G. barbadense) and Arabidopsis, Rice, Cacao, and Grapevine were searched with hmmsearch (v3.2.1) (E
Summary
The peroxiredoxin (PRX ) protein (EC 1.11.1.15) is a member of the thioredoxin-scaffold enzyme family and presents cysteine-dependent peroxidase activity against hydrogen peroxide substrates. Genomewide identification, evolution, expression, and alternative splicing profiles of peroxiredoxin genes in cotton. Research on this protein has mainly focused on mammalian disease resistance based on the loss of the protein, which leads to an imbalance in the cellular redox state (Oláhová et al, 2009). PRX is widely known to eliminate stress-induced excess free radicals in the body and protect cells from oxidative damage (Hofmann, Hecht & Flohé, 2002). PRX reduces the damage caused by oxidative stress and enhances the activity of natural killer cells (Nonn, Berggren & Powis, 2003), regulates cell proliferation, differentiation and apoptosis (Lee et al, 2003), and protects free radical-sensitive proteins (Wenders et al, 2003)
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