Abstract
Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca2+]cyt). Members of the Arabidopsis thaliana (L.) reduced hyperosmolality-gated calcium-permeable channels (OSCA) gene family are reported to be involved in sensing extracellular changes to trigger hyperosmolality-induced [Ca2+]cyt increases and controlling stomatal closure during immune signaling. Wheat (Triticum aestivum L.) is a very important food crop, but there are few studies of its OSCA gene family members. In this study, 42 OSCA members were identified in the wheat genome, and phylogenetic analysis can divide them into four clades. The members of each clade have similar gene structures, conserved motifs, and domains. TaOSCA genes were predicted to be regulated by cis-acting elements such as STRE, MBS, DRE1, ABRE, etc. Quantitative PCR results showed that they have different expression patterns in different tissues. The expression profiles of 15 selected TaOSCAs were examined after PEG (polyethylene glycol), NaCl, and ABA (abscisic acid) treatment. All 15 TaOSCA members responded to PEG treatment, while TaOSCA12/-39 responded simultaneously to PEG and ABA. This study informs research into the biological function and evolution of TaOSCA and lays the foundation for the breeding and genetic improvement of wheat.
Highlights
Publisher’s Note: MDPI stays neutralUnder natural conditions, plants encounter a variety of biotic and abiotic stresses [1,2].Plants resist these stresses by sensing and transmitting signals in a variety of ways that regulate responses and gene expression, thereby producing appropriate physiological and morphological changes [3,4,5]
Interferes with gene expression induced by drought, indicating that the precise regulation of OICI is essential for the activation of many signal transduction pathways triggered by external stimuli [7]
We identified the possible OSCA members in Triticum aestivum based on the criterion that the OSCA gene contains a conserved DUF221 functional domain
Summary
Plants encounter a variety of biotic and abiotic stresses [1,2]. Plants resist these stresses by sensing and transmitting signals in a variety of ways that regulate responses and gene expression, thereby producing appropriate physiological and morphological changes [3,4,5]. Plants induce a rapid increase in intracellular free Ca2+ concentration ([Ca2+ ]cyt ), thereby inducing the expression of many stress-related genes and regulating the tolerance of plants to osmotic stress [4,7]. Interferes with gene expression induced by drought, indicating that the precise regulation of OICI is essential for the activation of many signal transduction pathways triggered by external stimuli [7]. Previous studies have shown that osmotic/mechanical stimulus-gated calcium permeable channels with regard to jurisdictional claims in published maps and institutional affiliations
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