Genome-wide identification and expression patterns in response to signals from <italic>Phytophthora infestans </italic>of CrRLK1Ls gene family in potato

Abstract

<p id="C3">Catharanthus roseus receptor-like kinase1-like kinases (CrRLK1Ls), a class of plant-specific protein receptor kinases, play an important role in maintaining cell wall integrity, cell-to-cell communications, and biotic and abiotic stress response. In this study, StCrRLK1Ls family members were identified, and their physical and chemical characteristics, chromosome location, evolutionary characteristics, subcellular location, and the expression patterns were comprehensively analyzed. The results showed that 17 StCrRLK1Ls members were detected, with amino acid size (753-997 aa), molecular weight (83.34-108.69 kD), and isoelectric point (5.30-7.56). Among them, <italic>StCrRLK1Ls</italic> were mainly predicted on plasma membrane. Evolutionary analysis indicated that CrRLK1Ls from potato, rice, apple, <italic>Arabidopsis</italic>, and tomato could be divided into seven subgroups, and <italic>St</italic>CrRLK1Ls were distributed in subgroups III, IV, V, VI, and VII. Moreover, <italic>StCrRLK1Ls </italic>were distributed on eight chromosomes, and there are three tandem repeat gene clusters, including six members. In addition, there were many cis-elements in the promoter region of <italic>StCrRLK1Ls</italic>, which mainly responded to hormones, defense, and stress signals. After the inoculation with <italic>Phytophthora infestans</italic> (<italic>Pi</italic>), a large number of <italic>StCrRLK1Ls</italic> were differentially expressed in Atlantic and Longshu 7, and the relative expression levels of <italic>StCrRLK1L</italic>8 and <italic>StCrRLK1L</italic>10 were obviously up-regulated during <italic>Pi</italic> infection. The relative expression levels of <italic>StCrRLK1L</italic>11 were 9.74 times compared with the control at eight days Atlantic inoculated <italic>Pi</italic>. We speculated that they could be used as the candidate genes for disease resistance and further functional analysis, which might play an important role in response to pathogenic fungi of potato.