Genome-wide identification and expression analysis of the JAZ gene family in turnip

Kai Jia,Cunyao Yan,Huizhuan Yan,Jie Gao,Yunxia Cheng,Jing Zhang,Wenwen Li

doi:10.1038/s41598-021-99593-2

Abstract

JAZ is a plant-specific protein family involved in the regulation of plant development, abiotic stresses, and responses to phytohormone treatments. In this study, we carried out a bioinformatics analysis of JAZ genes in turnip by determining the phylogenetic relationship, chromosomal location, gene structure and expression profiles analysis under stresses. The 36 JAZ genes were identified and classified into four subfamilies (ZML, JAZ, PPD and TIFY). The JAZ genes were located on 10 chromosomes. Two gene pairs were involved in tandem duplication events. We identified 44 collinear JAZ gene pairs in the turnip genome. Analysis of the Ka/Ks ratios indicated that the paralogs of the BrrJAZ family principally underwent purifying selection. Expression analysis suggested JAZ genes may be involved in the formation of turnip tuberous root, and they also participated in the response to ABA, SA, MeJA, salt stress and low-temperature stress. The results of this study provided valuable information for further exploration of the JAZ gene family in turnip.

Highlights

Jasmonic acid and its biologically active derivatives are referred to as jasmonates (JAs), which participate in plant defenses to insects and contribute to developmental and growth controls[1,2,3]
JAZ gene family has many members that are involved in the regulation of plant development, abiotic stresses, and responses to phytohormone treatments, each with a different biological function[16]
36 and 37 homologous proteins were obtained according to the BLASTP search using 18 A. thaliana JAZ proteins and 36 B. oleracea JAZ proteins, respectively

Summary

Introduction

Jasmonic acid and its biologically active derivatives are referred to as jasmonates (JAs), which participate in plant defenses to insects and contribute to developmental and growth controls[1,2,3]. JAs induce the gene expression of signal transduction pathways, thereby regulating plant responses to a dversity[6]. The interaction of JAs with other phytohormones and the regulation of plant stress resistance has become a hot topic of research[7]. JAZ gene family has many members that are involved in the regulation of plant development, abiotic stresses, and responses to phytohormone treatments, each with a different biological function[16]. We used genomic resources to systematically identify members of the turnip JAZ gene family and investigated phylogeny, chromosome locations, evolutionary history, structural characteristics. We analyzed expression patterns of JAZ genes after different abiotic stresses and phytohormone treatments. This study will be useful for functional studies of JAZs in turnip

Methods

Results

Discussion

Conclusion