Genome-Wide Identification and Evolutionary Analysis of the SRO Gene Family in Tomato.

Ning Li,Baike Wang,Qinghui Yu,Juan Wang,Shaoyong Huang,Ruiqiang Xu,Jie Gao

doi:10.3389/fgene.2021.753638

Ning Li, Baike Wang + Show 5 more

Open Access

https://doi.org/10.3389/fgene.2021.753638

Copy DOI

Abstract

SRO (SIMILAR TO RCD ONE) is a family of plant-specific small molecule proteins that play an important role in plant growth and development and environmental responses. However, SROs still lack systematic characterization in tomato. Based on bioinformatics methods, SRO family genes were identified and characterized from cultivated tomatoes and several wild tomatoes. qRT-PCR was used to study the expression of SRO gene in cultivated tomatoes. Phylogenetic and evolutionary analyses showed that SRO genes in angiosperms share a common ancestor and that the number of SRO family members changed as plants diverged and evolved. Cultivated tomato had six SRO members, five of which still shared some degree of identity with the ancestral SRO genes. Genetic structure and physicochemical properties showed that tomato SRO genes were highly conserved with chromosomal distribution. They could be divided into three groups based on exon-intron structure, and cultivated tomato contained only two of these subclades. A number of hormonal, light and abiotic stress-responsive cis-regulatory elements were identified from the promoter of the tomato SRO gene, and they also interacted with a variety of stress-responsive proteins and microRNAs. RNA-seq analysis showed that SRO genes were widely expressed in different tissues and developmental stages of tomato, with significant tissue-specific features. Expression analysis also showed that SRO genes respond significantly to high temperature and salt stress and mediate the tomato hormone regulatory network. These results provide a theoretical basis for further investigation of the functional expression of tomato SRO genes and provide potential genetic resources for tomato resistance breeding.

Highlights

Plant growth and development are dynamic processes that interact with the surrounding environment
We suggested that an SRO genes that was triploidized in the ancestral species replicated in the genome-wide doubling event peculiar to the differentiation stage of Solanaceae and preserved in the evolutionary process, formed two members, SolySRO5 and SolySRO6, and tomato Chr6 and Chr8
Our study showed that SolySRO5 did have a direct interaction with PARP2 protein, which is the core member of the Poly (ADP-ribose) polymerase (PARP) family in plants (Song et al, 2015), It supported the possibility that SRO genes regulated active PARP proteins under specific conditions

Summary

Introduction

Plant growth and development are dynamic processes that interact with the surrounding environment. Environmental stress has always been one of the major factors limiting plant growth. The long evolutionary process has endowed plants with many means of coping with biotic and abiotic stresses. Transcription factors, as one of the main ways in which plants regulate their life activities, often play an important role in the plant stress response system (Nevo, 2001; Song et al, 2016). Many key stress response transcription factors have been identified in plants, such as MYB (Du et al, 2009), bHLH (Sun et al, 2018), and WRKY (Li et al, 2020).

Methods

Findings

Discussion

Conclusion