Abstract
The LOR (LURP-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against Hyaloperonospora parasitica (Hpa) in Arabidopsis. However, there are few reports of LOR genes in response to abiotic stresses in plants. In this study, a genome-wide survey and expression levels in response to abiotic stresses of 36 LOR genes from Glycine max were conducted. The results indicated that the GmLOR gene family was divided into eight subgroups, distributed on 14 chromosomes. A majority of members contained three extremely conservative motifs. There were four pairs of tandem duplicated GmLORs and nineteen pairs of segmental duplicated genes identified, which led to the expansion of the number of GmLOR genes. The expansion patterns of the GmLOR family were mainly segmental duplication. A heatmap of soybean LOR family genes showed that 36 GmLOR genes exhibited various expression patterns in different tissues. The cis-acting elements in promoter regions of GmLORs include abiotic stress-responsive elements, such as dehydration-responsive elements and drought-inducible elements. Real-time quantitative PCR was used to detect the expression level of GmLOR genes, and most of them were expressed in the leaf or root except that GmLOR6 was induced by osmotic and salt stresses. Moreover, GmLOR4/10/14/19 were significantly upregulated after PEG and salt treatments, indicating important roles in the improvement of plant tolerance to abiotic stress. Overall, our study provides a foundation for future investigations of GmLOR gene functions in soybean.
Highlights
The growth and productivity of plants are affected by adversely environmental factors, including drought, salinity, and extreme temperature [1,2,3,4,5]
A total of 36 GmLOR genes were obtained from the soybean genome (Wm82, a2.v1) in the Phytozome v12.1 database, and all of the putative protein sequences were submitted to
The Conserved Domain Database (CDD) to confirm the conserved LOR domain
Summary
The growth and productivity of plants are affected by adversely environmental factors, including drought, salinity, and extreme temperature [1,2,3,4,5]. Drought and salinity are majorly adverse environmental stresses that limit crop quality and yield in agriculture and threaten food security [6]. The LOR (LURP-one related) gene family contains a pfam 04525 domain (DUF567) defined by the Pfam database [7]. It is found in plants, fungi, eubacteria, and some archaea [8]. The LOR family had fifteen members in Arabidopsis thaliana [9].
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