Abstract
The U-box gene family is a family of genes which encode U-box domain-containing proteins. However, little is known about U-box genes in banana (Musa acuminata). In this study, 91 U-box genes were identified in banana based on its genome sequence. The banana U-box genes were distributed across all 12 chromosomes at different densities. Phylogenetic analysis of U-box genes from banana, Arabidopsis, and rice suggested that they can be clustered into seven subgroups (I–VII), and most U-box genes had a closer relationship between banana and rice relative to Arabidopsis. Typical U-box domains were found in all identified MaU-box genes through the analysis of conserved motifs. Four conserved domains were found in major banana U-box proteins. The MaU-box gene family had the highest expression in the roots at the initial fruit developmental stage. The MaU-box genes exhibited stronger response to drought than to salt and low temperatures. To the best of our knowledge, this report is the first to perform genome-wide identification and analysis of the U-box gene family in banana, and the results should provide valuable information for better understanding of the function of U-box in banana.
Highlights
The ubiquitin/26S proteasome (UPS) pathway degrades ubiquitinated substrate proteins and is extensively involved in various cellular processes [1]
Inactivation of the Arabidopsis PUB13 results in spontaneous cell death, enhanced levels of the defence hormone SA, and early flowering [23]
91 Plant U-box (PUB) genes are found in banana genome (Table 1)
Summary
The ubiquitin/26S proteasome (UPS) pathway degrades ubiquitinated substrate proteins and is extensively involved in various cellular processes [1]. The diverse aspects of plant growth and development and the degradation of short-lived regulatory proteins can be regulated by the UPS [2,3,4]. E1 activates the ubiquitin molecule in an ATP-dependent manner, and E2 facilitates the attachment of ubiquitin molecule to the target protein in the presence of E3 [6]. E3 ligase plays an important role in protein ubiquitination because E3 can identify target proteins for modification [7]. Ubiquitin E3 ligases facilitate the covalent attachment of ubiquitin to target proteins in eukaryotes [10]. The genome of Arabidopsis thaliana has more than 60 U-box genes, which have many functions in plants [16]. Inactivation of the Arabidopsis PUB13 results in spontaneous cell death, enhanced levels of the defence hormone SA, and early flowering [23]
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