Genome-wide identification and analysis of the sucrose synthase gene family in cassava (Manihot esculenta Crantz).

Abstract

Sucrose synthase (SUS), a key enzyme of the sucrose metabolism pathway, is encoded by a multi-gene family in plants. To date, dozens of SUS gene families have been characterized in various plant genomes. However, only a few studies have performed comprehensive analyses in tropical crops like cassava (Manihot esculenta Crantz). In the present study, seven non-redundant members of the SUS gene family (MeSUS1-7) were identified and characterized from the cassava genome. The MeSUS genes were distributed on five chromosomes (Chr1, Chr2, Chr3, Chr14, and Chr16) and the encoded proteins could be classified into three major groups with other SUS proteins from both dicot and monocot species (SUS I, SUS II, and SUS III). The spatio-temporal expression profiles of MeSUS genes showed a developmental stage-dependent, partially overlapping pattern, mainly expressed in the source and sink tissues. Cold and drought treatments significantly induced the expressions of MeSUS2, MeSUS4, MeSUS6, and MeSUS7 and the activities of the encoded enzymes, indicating that these genes may play crucial roles in resistance against abiotic stresses. These results provide new insights into the multifaceted role of the SUS gene family members in various physiological processes, especially sucrose transport and starch accumulation in cassava roots.