Genome Wide Identification and Analysis of <i>TRK</i> Gene Family in <i>Aspergillus niger</i

Abstract

TRK gene is related to potassium uptake and transport, which plays an important role in regulating the life activity of living organisms. Based on Aspergillus niger genome database, using bioinformatic method predicted the members of TRK gene family, gene structure and phylogenetic relationships of Aspergillus nig er. The phylogenetic tree was constructed using MEGA 6.0.6 software with Maximum Likelihood Tree method. Using PAL2NAL software estimated da/ds. The structure of the genes was analyzed by GSDS 2.0 software. The motif was analyzed by MEME program. Four members of TRK gene family in the genome of Aspergillus niger were identified, which were AnTrk1 , AnTrk2 , AnTrk3 and AnTrk4 , respectively. Both the TRK feature domain and the conserved motif were found. The structure of different genes was different, but the structure of cluster was similar. TRK gene family members of Aspergillus niger in the genetic structure and other aspects of existed significant differences with the yeast. Genetic relationships and protein interaction network analysis have further verified this conclusion. Therefore, it can infer that TRK gene of Aspergillus niger has other regulation pathways. This conclusion could provide the basis for further research on the function and mechanism of TRK osmotic stress related protein, so as to lay a foundation for understanding the mechanism of the osmotic adjustment of Aspergillus niger .