Abstract
The basic leucine zipper (bZIP) regulates plant growth and responds to stress as a key transcription factor of the Abscisic acid (ABA) signaling pathway. In this study, TabZIP genes were identified in wheat and the gene structure, physicochemical properties, cis-acting elements, and gene collinearity were analyzed. RNA-Seq and qRT-PCR analysis showed that ABA and abiotic stress induced most TabZIP genes expression. The ectopic expression of TaABI5 up-regulated the expression of several cold-responsive genes in Arabidopsis. Physiological indexes of seedlings of different lines under freezing stress showed that TaABI5 enhanced the freezing tolerance of plants. Subcellular localization showed that TaABI5 is localized in the nucleus. Furthermore, TaABI5 physically interacted with cold-resistant transcription factor TaICE1 in yeast two-hybrid system. In conclusion, this study identified and analyzed members of the TabZIP gene family in wheat. It proved for the first time that the gene TaABI5 affected the cold tolerance of transgenic plants and was convenient for us to understand the cold resistance molecular mechanism of TaABI5. These results will provide a new inspiration for further study on improving plant abiotic stress resistance.
Highlights
Wheat is one of the most important crops and is an important plant protein resource around the world
All genes were induced under Abscisic acid (ABA), and the expression trend was similar to that under JA treatment. These results suggest that TabZIP genes may play a pivotal role in these two hormone pathways, and JA responsive genes are regulated by basic leucine zipper (bZIP) transcription factors [78]
A total of 227 TabZIP genes were identified in wheat, adding 36 new genes from previous studies
Summary
Wheat is one of the most important crops and is an important plant protein resource around the world. Chilling stress and freezing stress can damage cell membranes and increase ROS in the body, affecting the growth and physiological state of plants, especially the yield and quality of wheat and other field crops [1]. Plant transcription factors (TFs) play crucial roles in the regulatory and biological processes under various environmental stresses [3]. TFs are sequence-specific binding proteins that bind to the promoter regions of specific target genes to regulate their transcription [4,5]. It controls cell processes such as signal transduction, cell morphogenesis and resistance to environmental stress in plants [6,7]
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