Abstract
Puccinia triticina (Pt), the causal agent of wheat leaf rust, is one of the most destructive fungal pathogens threatening global wheat cultivations. The rational utilization of leaf rust resistance (Lr) genes is still the most efficient method for the control of such diseases. The Lr47 gene introgressed from chromosome 7S of Aegilops speltoides still showed high resistance to the majority of Pt races collected in China. However, the Lr47 gene has not been cloned yet, and the regulatory network of the Lr47-mediated resistance has not been explored. In the present investigation, transcriptome analysis was applied on RNA samples from three different wheat lines (“Yecora Rojo”, “UC1037”, and “White Yecora”) carrying the Lr47 gene three days post-inoculation with the epidemic Pt race THTT. A comparison between Pt-inoculated and water-inoculated “Lr47-Yecora Rojo” lines revealed a total number of 863 upregulated (q-value < 0.05 and log2foldchange > 1) and 418 downregulated (q-value < 0.05 and log2foldchange < −1) genes. Specifically, differentially expressed genes (DEGs) located on chromosomes 7AS, 7BS, and 7DS were identified, ten of which encoded receptor-like kinases (RLKs). The expression patterns of these RLK genes were further determined by a time-scale qRT-PCR assay. Moreover, heatmaps for the expression profiles of pathogenesis-related (PR) genes and several transcription factor gene families were generated. Using a transcriptomic approach, we initially profiled the transcriptional changes associated with the Lr47-mediated resistance. The identified DEGs, particularly those genes encoding RLKs, might serve as valuable genetic resources for the improvement of wheat resistance to Pt.
Highlights
Puccinia triticina (Pt), the causal agent of wheat leaf rust, is a wide-spread fungal pathogen affecting global wheat production
Aas saucsocnetprtoilb.leCownhsiedaetrlainblee“sTphorautclahteior”n wofitPhtowutasaonbysLerrved in gtheenessussecrevpetdibales alinceon8trdopl.iC, ownhseidreearsabwlehsepaot rluinlaetsiocnarorfyPintgwtahseoLbrs4e7rvgeednien sthhoewsuesdcenpetcibroletilcinsepots phe8ndoptyi,pweh(Ferigeausrew1h).eat lines carrying the Lr47 gene showed necrotic spots phenotype (Figure 1)
We observed a broad range of transcripts encoding receptor-like kinases (RLKs) and Cytochrome P450 in the identified upregulated differentially expressed genes (DEGs), whereas several heat-stress-responsive genes were significantly suppressed during the Lr47-mediated resistance
Summary
Puccinia triticina (Pt), the causal agent of wheat leaf rust, is a wide-spread fungal pathogen affecting global wheat production. APR normally show durable and broad-spectrum resistance to multiple fungal diseases, including yellow rust (Yr), leaf rust (Lr), stem rust (Sr), and powdery mildew (Pm). It is time-consuming and labor-intensive for the genetic cloning of quantitative trait loci (QTL) controlling the APR. Transcript reads were assembled using the reference genome of Triticum aestivum (AABBDD, “Chinese Spring” TGACv1 version) to determine the DEGs regulated by the Lr47 gene during the wheat resistance to Pt infection. The genome-wide expression patterns of genes associated with the Lr47-mediated resistance was initially profiled, which would provide valuable genetic resources for the improvement of wheat resistance to Pt. Int.vJ.aMluoal.bSlcei.g20e1n9e, t2i0c, 4r4e9s8ources for the improvement of wheat resistance to Pt
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