Abstract
Background and Aims : Cholesterol is a fundamental component for cellular survival. LDL cholesterol is transported into the cell via endocytosis by the LDL receptor. Cholesterol is released in lysosomes and transported to other cellular structures. Not all proteins involved in this transport to the ER are identified. Therefore, we aimed to set up a genome-wide CRISPR-Cas9 screen to identify unknown proteins involved in intracellular cholesterol transport.
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