Abstract
Pleurotus ostreatus is one of the most widely cultivated mushrooms in China. The regulatory mechanisms of fruiting body formation and the response to heat stress in P. ostreatus are main research focuses. The Zn(II)2Cys6 family is one of the largest families of transcriptional factors and plays important roles in multiple biological processes in fungi. In this study, we identified 66 zinc cluster proteins in P. ostreatus (PoZCPs) through a genome-wide search. The PoZCPs were classified into 15 types according to their zinc cluster domain. Physical and chemical property analyses showed a huge diversity among the PoZCPs. Phylogenetic analysis of PoZCPs classified these proteins into six groups and conserved motif combinations and similar gene structures were observed in each group. The expression profiles of these PoZCP genes during different developmental stages and under heat stress were further investigated by RNA-sequencing (RNA-seq), revealing diverse expression patterns. A total of 13 PoZCPs that may participate in development or the heat stress response were selected for validation of their expression levels through real-time quantitative PCR (RT-qPCR) analysis, and some developmental stage-specific and heat stress-responsive candidates were identified. The findings contribute to our understanding of the roles and regulatory mechanisms of ZCPs in P. ostreatus.
Highlights
Pleurotus ostreatus is a widely cultivated mushroom in China because of its high nutritional value, uncomplicated cultivation techniques, and broad adaptability (Miles & Chang, 2004; Khan & Tania, 2012)
Identification of zinc cluster protein (ZCP) genes in P. ostreatus After manual correction, a total of 66 unique genes with conserved zinc cluster domains were identified as ZCP genes in P. ostreatus, accounting for 23.82% of all transcription factors
The 66 genes were named PoZCP01 to PoZCP66, and the coding sequences were submitted to GenBank under accession numbers MN652925–MN652990
Summary
Pleurotus ostreatus is a widely cultivated mushroom in China because of its high nutritional value, uncomplicated cultivation techniques, and broad adaptability (Miles & Chang, 2004; Khan & Tania, 2012). Dehydration responsive element binding protein (DREB) improves heat resistance in A. thaliana and Nicotiana tabacum (Sakuma et al, 2006; Arroyo-Herrera et al, 2016) Transcription factors such as those of the NAC (Shahnejat-Bushehri, Mueller-Roeber & Balazadeh, 2012; Fang et al, 2015; Guo et al, 2015), MYB (El-Kereamy et al, 2012; Casaretto et al, 2016), WRKY (Li et al, 2009; Cai et al, 2015; He et al, 2016) and zinc finger (Kim, Cho & Yoo, 2015; Koguchi et al, 2017; Agarwal & Khurana, 2018) families are involved in tolerance to high temperature in many eukaryotes
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