Abstract
BackgroundThe plant-specific homeodomain-leucine zipper class IV (HD-ZIP IV) gene family has been involved in the regulation of epidermal development.ResultsFifteen genes coding for HD-ZIP IV proteins were identified (NtHD-ZIP-IV-1 to NtHD-ZIP-IV-15) based on the genome of N. tabacum. Four major domains (HD, ZIP, SAD and START) were present in these proteins. Tissue expression pattern analysis indicated that NtHD-ZIP-IV-1, − 2, − 3, − 10, and − 12 may be associated with trichome development; NtHD-ZIP-IV-8 was expressed only in cotyledons; NtHD-ZIP-IV-9 only in the leaf and stem epidermis; NtHD-ZIP-IV-11 only in leaves; and NtHD-ZIP-IV-15 only in the root and stem epidermis. We found that jasmonates may induce the generation of glandular trichomes, and that NtHD-ZIP-IV-1, − 2, − 5, and − 7 were response to MeJA treatment. Dynamic expression under abiotic stress and after application of phytohormones indicated that most NtHD-ZIP IV genes were induced by heat, cold, salt and drought. Furthermore, most of these genes were induced by gibberellic acid, 6-benzylaminopurine, and salicylic acid, but were inhibited by abscisic acid. NtHD-ZIP IV genes were sensitive to heat, but insensitive to osmotic stress.ConclusionNtHD-ZIP IV genes are implicated in a complex regulatory gene network controlling epidermal development and abiotic stress responses. The present study provides evidence to elucidate the gene functions of NtHD-ZIP IVs during epidermal development and stress response.
Highlights
The plant-specific homeodomain-leucine zipper class homeodomain-leucine zipper class IV family (IV) (HD-ZIP IV) gene family has been involved in the regulation of epidermal development
Identification and analysis of HD-ZIP IV genes in N. tabacum Based on the latest genome data of tobacco, 32 HD-ZIP IV genes were identified in N. tabacum genomes
The results indicate that most NtHD-ZIP IVs were sensitive to heat, but Conclusions Fifteen HD-ZIP IV genes were identified from N. tabacum genome
Summary
The plant-specific homeodomain-leucine zipper class IV (HD-ZIP IV) gene family has been involved in the regulation of epidermal development. Plant trichomes are involved in defense responses towards insect predation, UV damage, toxin sequestration, and excess transpiration. Trichomes are grouped into two types, glandular and non-glandular. The plant-specific homeodomain-leucine zipper (HD-Zip) gene family plays a crucial role in abiotic stress response and plant development [11,12,13]. These proteins can be further grouped into 4 subfamilies according to their structural features, conserved domains, and physiological functions [[14,15,16,17].
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