Abstract
Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H2O2 homeostasis and normalize plant reaction to abiotic stress conditions. To understand the major roles of the GPX gene family in rapeseed (Brassica napus L.), for the first time, a genome-wide study identified 25 BnGPX genes in the rapeseed genome. The phylogenetic analysis discovered that GPX genes were grouped into four major groups (Group I–Group IV) from rapeseed and three closely interrelated plant species. The universal investigation uncovered that the BnGPXs gene experienced segmental duplications and positive selection pressure. Gene structure and motifs examination recommended that most of the BnGPX genes demonstrated a comparatively well-maintained exon-intron and motifs arrangement within the identical group. Likewise, we recognized five hormones-, four stress-, and numerous light-reactive cis-elements in the promoters of BnGPXs. Five putative bna-miRNAs from two families were also prophesied, targeting six BnGPXs genes. Gene ontology annotation results proved the main role of BnGPXs in antioxidant defense systems, ROS, and response to stress stimulus. Several BnGPXs genes revealed boosted expression profiles in many developmental tissues/organs, i.e., root, seed, leaf, stem, flower, and silique. The qRT-PCR based expression profiling exhibited that two genes (BnGPX21 and BnGPX23) were suggestively up-regulated against different hormones (ABA, IAA, and MeJA) and abiotic stress (salinity, cold, waterlogging, and drought) treatments. In short, our discoveries provide a basis for additional functional studies on the BnGPX genes in future rapeseed breeding programs.
Highlights
IntroductionPlants are frequently exposed to several environmental stresses, comprising both abiotic and biotic, which significantly affect the growth and developmental processes [1,2,3,4]
Based on BLASTP examinations against the rapeseed genome employing eight Arabidopsis AtGPXs proteins as enquiries, a total of 25 presumed glutathione peroxidases (GPXs) genes were recognized in the complete rapeseed genome (Table 1)
Theconfirms miRNA-targeted areare shown in Figure with cellular anatomical entity (GO:0005737), obsolete intracel6B, and the comprehensive data(GO:0110165), of all miRNAscytoplasm targeted sites/genes are introduced in Talular part (GO:0044424), obsolete cell part (GO:0044464), and organelle (GO:0043226), etc
Summary
Plants are frequently exposed to several environmental stresses, comprising both abiotic and biotic, which significantly affect the growth and developmental processes [1,2,3,4]. These traumas can boost the production of reactive oxygen species (ROS), impairing cellular apparatuses and macromolecules, including DNA, proteins, and lipids, and eventually. In comparison to mammals GPXs, plants GPXs have a partiality to Trx as a replacement for GSH as the dipping substratum [10,11]. Plants’ GPXs comprise cysteine (Cys) in their functioning sites, whereas usually, mammals’ GPXs comprise selenocysteine (SeCys) residue as a substitute to Cys [9,11,12]
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