Abstract
BackgroundCesA and Csl gene families, which belong to the cellulose synthase gene superfamily, plays an important role in the biosynthesis of the plant cell wall. Although researchers have investigated this gene superfamily in several model plants, to date, no comprehensive analysis has been conducted in the common bean.ResultsIn this study, we identified 39 putative cellulose synthase genes from the common bean genome sequence. Then, we performed a bioinformatics analysis of this gene family involving sequence alignment, phylogenetic analysis, gene structure, collinearity analysis and chromosome location. We found all members possess a cellulose_synt domain. Phylogenetic analysis revealed that these cellulose synthase genes may be classified into five subfamilies, and that members in the same subfamily share conserved exon-intron distribution and motif compositions. Abundant and distinct cis-acting elements in the 2 k basepairs upstream regulatory regions indicate that the cellulose synthase gene family may plays a vital role in the growth and development of common bean. Moreover, the 39 cellulose synthase genes are distributed on 10 of the 11 chromosomes. Additionally expression analysis shows that all CesA/Csl genes selected are constitutively expressed in the pod development.ConclusionsThis research reveals both the putative biochemical and physiological functions of cellulose synthase genes in common bean and implies the importance of studying non-model plants to understand the breadth and diversity of cellulose synthase genes.
Highlights
CesA and Cellulose synthase-like (Csl) gene families, which belong to the cellulose synthase gene superfamily, plays an important role in the biosynthesis of the plant cell wall
We searched for the cellulose synthase gene family of common bean using the constructed model and a total of 39 sequences can matched to CesA/Csl superfamily. 14 gene members contained a cellulose synthase domain (CS) and zinc finger structure, 25 gene members only harbored a CS domain
The results showed that all 21 CesA/Csl genes were expressed in all three pod development stages, suggesting that all these genes are necessary for the pod growth
Summary
CesA and Csl gene families, which belong to the cellulose synthase gene superfamily, plays an important role in the biosynthesis of the plant cell wall. There exists a cellulose synthase superfamily including CesA (cellulose synthase) and Csl (cellulose synthase-like) gene family, both of which belong to the glycosyltransferase GT2 family and have a similar protein sequence structure. The cellulose synthase (CesA) gene was first identified from cotton fibers according to its sequence similarity with a bacterial CesA gene [3]. Cellulose synthase genes were identified in Arabidopsis [4], rice [5], and maize [6], barley [7]. In Arabidopsis thaliana, AtCesA1, AtCesA3, and AtCesA6 form a cellulose synthase complex and participate in the synthesis of the primary cell walls. Liu et al BMC Genomic Data (2022) 23:9
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