Abstract
The content of chlorophyll, a fundamental component required for photosynthesis in plants, has been widely studied across crop species. In this study, we aimed to evaluate the genetic diversity of 453 peanut accessions. We evaluated the evolutionary relationships using a genome-wide association study (GWAS) of leaf color data based on chlorophyll content analysis using the Axiom_Arachis array containing 58K single-nucleotide polymorphisms (SNPs). We identified seven SNPs as being significantly associated with leaf chlorophyll content on the chromosomes Aradu.A02, Aradu.A08, Araip.B02, Araip.B05, Araip.B06, and Araip.B08 in a GAPIT analysis. The SNP AX-176820297 on Araip.B05 was significantly linked with leaf chlorophyll content across the seasons. The Arahy.SDG4EV gene was detected to be in linkage disequilibrium (LD) with the significant SNPs, and its expression was significantly correlated with leaf chlorophyll content. The results of the current study provide useful and fundamental information with which to assess genetic variations in chlorophyll content and can be utilized for further genetic and genomic studies and breeding programs in peanuts.
Highlights
IntroductionThe content of chlorophyll, a fundamental component required for photosynthesis in plants, has been widely studied across crop species
After eliminating single-nucleotide polymorphisms (SNPs) with high levels of missing data (>20%), heterozygosity (>20%), or low minor allele frequency (MAF) (
Our study demonstrated the feasibility of genomewide association study (GWAS) analysis using the core germplasm and a high-density array chip to study leaf chlorophyll content in the peanut (Supplementary Figure S2)
Summary
The content of chlorophyll, a fundamental component required for photosynthesis in plants, has been widely studied across crop species. We aimed to evaluate the genetic diversity of 453 peanut accessions. We evaluated the evolutionary relationships using a genomewide association study (GWAS) of leaf color data based on chlorophyll content analysis using the. SNPs as being significantly associated with leaf chlorophyll content on the chromosomes Aradu.A02, Aradu.A08, Araip.B02, Araip.B05, Araip.B06, and Araip.B08 in a GAPIT analysis. The SNP AX176820297 on Araip.B05 was significantly linked with leaf chlorophyll content across the seasons. SNPs, and its expression was significantly correlated with leaf chlorophyll content. The results of the current study provide useful and fundamental information with which to assess genetic variations in chlorophyll content and can be utilized for further genetic and genomic studies and breeding programs in peanuts.
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