Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

Yucheng Zheng,Pengjie Wang,Yun Sun,Chuan Yue,Xuejin Chen,Naixing Ye

doi:10.1038/s41598-020-59675-z

Yucheng Zheng, Pengjie Wang + Show 4 more

Open Access

https://doi.org/10.1038/s41598-020-59675-z

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Journal: Scientific Reports	Publication Date: Feb 17, 2020
Citations: 23	License type: open-access

Affiliation: Fujian Agriculture and Forestry University

Abstract

The JASMONATE-ZIM DOMAIN (JAZ) family genes are key repressors in the jasmonic acid signal transduction pathway. Recently, the JAZ gene family has been systematically characterized in many plants. However, this gene family has not been explored in the tea plant. In this study, 13 CsJAZ genes were identified in the tea plant genome. Phylogenetic analysis showed that the JAZ proteins from tea and other plants clustered into 11 sub-groups. The CsJAZ gene transcriptional regulatory network predictive and expression pattern analyses suggest that these genes play vital roles in abiotic stress responses, phytohormone crosstalk and growth and development of the tea plant. In addition, the CsJAZ gene expression profiles were associated with tea postharvest processing. Our work provides a comprehensive understanding of the CsJAZ family and will help elucidate their contributions to tea quality during tea postharvest processing.

Highlights

The JASMONATE-ZIM DOMAIN (JAZ) family genes are key repressors in the jasmonic acid signal transduction pathway
The grand average of hydropathicity (GRAVY) values of all CsJAZ proteins ranged from −0.916 to −0.310, indicating that the 13 CsJAZ proteins were hydrophilic, with GRAVY values less than zero (Table 1)
Some cis-elements related to JA response exist in the upstream or downstream of the CsJAZ gene may be responsible for the gene activation.Interestingly, we found that AtJAZ1-2 and CsJAZ12 clustered into the same subclade, AtJAZ7-8 clustered with CsJAZ7, AtJAZ9 clustered with CsJAZ1 and AtJAZ10 clustered with CsJAZ13 and CsJAZ8

Summary

Introduction

The JASMONATE-ZIM DOMAIN (JAZ) family genes are key repressors in the jasmonic acid signal transduction pathway. The CsJAZ gene transcriptional regulatory network predictive and expression pattern analyses suggest that these genes play vital roles in abiotic stress responses, phytohormone crosstalk and growth and development of the tea plant. The JAZ protein family contains the highly conserved TIFY domain (ZIM) with the consensus sequence TIF[F/Y]XG at the N-terminus and the Jas domain (CCT_2) with the consensus sequence SLX2FX2KRX2RX5PY at the C-terminus[12] These two core highly conserved domains play irreplaceable roles in the JA signal transduction. The TIFY domain has been found to interact with NINJA, which is a novel interactor with JAZ, and these genes play a joint inhibitory role in the JA signal transduction pathway[18]. Research has indicated that loss of the Jas domain confers insensitivity to plant resistance to insects[19]

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