Abstract
JAZ (Jasmonate ZIM-domain) proteins play pervasive roles in plant development and defense reaction. However, limited information is known about the JAZ family in Camellia sinensis. In this study, 12 non-redundant JAZ genes were identified from the tea plant genome database. Phylogenetic analysis showed that the 12 JAZ proteins belong to three groups. The cis-elements in promoters of CsJAZ genes and CsJAZ proteins interaction networks were also analyzed. Quantitative RT–PCR analysis showed that 7 CsJAZ genes were preferentially expressed in roots. Furthermore, the CsJAZ expressions were differentially induced by cold, heat, polyethylene glycol (PEG), methyl jasmonate (MeJA), and gibberellin (GA) stimuli. The Pearson correlations analysis based on expression levels showed that the CsJAZ gene pairs were differentially expressed under different stresses, indicating that CsJAZs might exhibit synergistic effects in response to various stresses. Subcellular localization assay demonstrated that CsJAZ3, CsJAZ10, and CsJAZ11 fused proteins were localized in the cell nucleus. Additionally, the overexpression of CsJAZ3, CsJAZ10, and CsJAZ11 in E. coli enhanced the growth of recombinant cells under abiotic stresses. In summary, this study will facilitate the understanding of the CsJAZ family in Camellia sinensis and provide new insights into the molecular mechanism of tea plant response to abiotic stresses and hormonal stimuli.
Highlights
Plants regulate their growth and developmental processes to adapt to various internal and external stimuli [1]
We examined the subcellular localizations of three JAZ genes, CsJAZ3, CsJAZ10, and CsJAZ11 in onion cells, and growth conditions of overexpression cells of these three genes under the abiotic stresses
The expression patterns of the CsJAZ genes were detected by qRT–PCR, and the results revealed obvious differences under low temperature, high temperature, water deficit (10% polyethylene glycol (PEG) 6000), and methyl jasmonate (MeJA) and GA treatments with different exposure time
Summary
Plants regulate their growth and developmental processes to adapt to various internal and external stimuli [1]. It has been reported that the JA signaling molecules, SCFCOI1 complex, Jasmonate-ZIM (JAZ) domain repressor, and MYC2 transcription activator are involved in the JA signaling pathway and could interact with each other during the JA signaling process [8]. The COI1–JAZ–MYC2 model has been considered as the first central signal module in the JA pathway In another model, the JAZ proteins recruit the repressors TPL (TOPLESS) and TPRs (TPL-related proteins) via an adaptor protein NINJA (novel interactor of JAZ) to repress the expressions of transcription factors (TFs) in the absence of the stimulation. JAZ proteins are involved in other plant hormone biosynthesis or signaling pathways such as gibberellic acid, auxin, ethylene, and salicylic acid (SA) [12]. Thereby JAZ proteins are involved in the repression of multiple TFs and inactivate their downstream responses, respectively [7,12,13]
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