Abstract
Rapid and dynamic changes in the expression of small RNAs are seen during ovule and fiber development in allotetraploid cotton.
Highlights
Cotton fiber development undergoes rapid and dynamic changes in a single cell type, from fiber initiation, elongation, primary and secondary wall biosynthesis, to fiber maturation
Distribution of small RNAs in cotton To characterize small RNAs in cotton, we made four barcoded sequencing libraries using total RNAs extracted from leaves, immature ovules (3 days prior to anthesis, -3 DPA), ovules with fiber cell initials, and young fiber-bearing ovules (3 days post-anthesis, +3 DPA) in Gossypium hirsutum L. cv
After removal of adaptor sequences, we identified the reads matching known cellular small RNAs, mitochondrial, and plastid sequences
Summary
Cotton fiber development undergoes rapid and dynamic changes in a single cell type, from fiber initiation, elongation, primary and secondary wall biosynthesis, to fiber maturation. Previous studies showed that cotton genes encoding putative MYB transcription factors and phytohormone responsive factors were induced during early stages of ovule and fiber development. Many of these factors are targets of microRNAs (miRNAs) that mediate target gene regulation by mRNA degradation or translational repression. Cotton fiber cell initiation and elongation are directly affected by plant phytohormones. Cotton genes encoding putative MYB transcription factors are induced during early stages of fiber development but repressed in a naked seed mutant that is impaired in fiber formation [3,7]. Many genes encoding putative transcription and phytohormone responsive factors are targets of microRNAs (miRNAs)
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