Abstract
The plant-specific transcription factor gene family, YABBY, belongs to the subfamily of zinc finger protein superfamily and plays an essential regulatory role in lateral organ development. In this study, nine YABBY genes were identified in the pineapple genome. Seven of them were located on seven different chromosomes and the remaining two were located on scaffold 1235. Through protein structure prediction and protein multiple sequence alignment, we found that AcYABBY3, AcYABBY5 and AcYABBY7 lack a C2 structure in their N-terminal C2C2 zinc finger protein structure. Analysis of the cis-acting element indicated that all the seven pineapple YABBY genes contain multiple MYB and MYC elements. Further, the expression patterns analysis using the RNA-seq data of different pineapple tissues indicated that different AcYABBYs are preferentially expressed in various tissues. RT-qPCR showed that the expression of AcYABBY2, AcYABBY3, AcYABBY6 and AcYABBY7 were highly sensitive to abiotic stresses. Subcellular localization in pineapple protoplasts, tobacco leaves and Arabidopsis roots showed that all the seven pineapple YABBY proteins were nucleus localized. Overexpression of AcYABBY4 in Arabidopsis resulted in short root under NaCl treatment, indicating a negative regulatory role of AcYABBY4 in plant resistance to salt stress. This study provides valuable information for the classification of pineapple AcYABBY genes and established a basis for further research on the functions of AcYABBY proteins in plant development and environmental stress response.
Highlights
Plants are often exposed to extreme environments during their development and growth
According to the mapping results, seven AcYABBY genes were localized on seven different chromosomes and two genes were located on scaffold1235 (Figure 1)
We found that some pineapple YABBY genes, including AcYABBY3, AcYABBY5 and AcYABBY7, lack a N-terminal C2C2 zinc finger domain
Summary
Plants are often exposed to extreme environments during their development and growth Abiotic stresses such as salt, drought, high temperature and cold lead to adverse effect on growth and development of plants, resulting in yield loss. Transcription factors play a crucial role in plant defense system regulating gene expression, some of which are associated with the abiotic stress response [1]. Transcription factors are divided into four functional regions, namely DNA binding domain, oligomeric site, transcriptional regulatory domain and nuclear localization signal region. These functional domains determine the characteristics, function, regulation and nuclear localization of transcription factors. The amino acid residues in these two domains are highly conserved and these domains are involved in the specific binding of DNA [2]
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