Abstract
Genes of the plant-specific YABBY transcription factor family have various roles, including lateral organ development, establishment of dorsoventral polarity, and response to abiotic stress. In this study, we carried out a genomic census of YABBY genes in grapevine (Vitis vinifera) and characterized their expression pattern during ovule development. We identified seven YABBY genes and classified them into five subfamilies, based on peptide sequence, similarity of exon–intron structure and composition of peptide sequence motifs. Analysis of YABBY gene expression in various grapevine structures and organs suggested that these genes function in diverse aspects of development and physiology. Analysis of expression during ovule development in four cultivars showed that one gene, VvYABBY4, was preferentially expressed during the period of ovule abortion in seedless cultivars. Transgenic expression of VvYABBY4 in tomato conferred reduced plant stature, dark green leaves, elongated pistil, and reduced size of fruit and seeds. Reduced seed size was associated with smaller endosperm cells. Expression of VvYABBY4 also affected expression of numerous tomato genes with presumed roles in seed development. These data suggest the potential for VvYABBY4 to influence seed development in grapevine, which may impact seedless grape breeding.
Highlights
The plant-specific YABBY transcription factors play important roles in the establishment of adaxial– abaxial polarity (Kumaran et al, 2002), development of lateral organs (Bowman et al, 1989), and stress response (Zhao et al, 2017)
Based on the presence of an Openreading frames (ORFs) encoding a single, conserved YABBY domain, we identified seven YABBY genes in a draft genome sequence of grapevine
The YABBY family of plant-specific transcription factor plays an important role in abaxial–adaxial polarity and development of lateral organs
Summary
The plant-specific YABBY transcription factors play important roles in the establishment of adaxial– abaxial polarity (Kumaran et al, 2002), development of lateral organs (Bowman et al, 1989), and stress response (Zhao et al, 2017). The genome of Arabidopsis thaliana (Arabidopsis) encodes for six YABBY members, which have been classified into five subfamilies, designated FIL/YAB3, CRC, INO, YAB2, and YAB5. FILAMENTOUS FLOWER (FIL), YAB3, YAB2, and YAB5 act redundantly to promote lateral organ development. INNER NO OUTER (INO) plays a seemingly distinct role to VvYABBY Family and Function of VvYABBY4 promote development of the ovule outer integument, the layer of cells surrounding the nucleus, into the seed coat (Villanueva et al, 1999; Bowman, 2000)
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