Abstract
Trihelix transcription factors play important roles in plant growth, development and various stress responses. In this study, we identified 32 trihelix family genes (DoGT) in the important Chinese medicinal plant Dendrobium officinale. These trihelix genes could be classified into five different subgroups. The gene structure and conserved functional domain of these trihelix genes were similar in the same subfamily but diverged between different subfamilies. Various stresses responsive cis-elements presented in the promoters of DoGT genes, suggesting that the trihelix genes might respond to the environmental stresses. Expressional changes of DoGT genes in three tissues and under cold treatment suggested that trihelix genes were involved in diverse functions during D. officinale development and cold tolerance. This study provides novel insights into the phylogenetic relationships and functions of the D. officinaletrihelix genes, which will aid future functional studies investigating the divergent roles of trihelix genes belonging to other species.
Highlights
IntroductionThese trihelix genes could be classified into five different subgroups
Bio-Innovation Center of DR PLANT, Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Citation: Tong, Y.; Huang, H.; Wang, Abstract: Trihelix transcription factors play important roles in plant growth, development and various stress responses
In order to identify the trihelix gene family, firstly, we downloaded the genome sequences of D. officinale from NCBI (http://www.ncbi.nlm.nih.gov/)
Summary
These trihelix genes could be classified into five different subgroups. The gene structure and conserved functional domain of these trihelix genes were similar in the same subfamily but diverged between different subfamilies. Various stresses responsive cis-elements presented in the promoters of DoGT genes, suggesting that the trihelix genes might respond to the environmental stresses. Expressional changes of DoGT genes in three tissues and under cold treatment suggested that trihelix genes were involved in diverse functions during. TFs activate or inhibit transcription by combined with gene promoter regions and cis-acting elements [2,3]. There are differences between clades, the internal hydrophobic region of each α helix of each trihelix domain in GT-1 and SH4 contains a tryptophan residue [8]. The most notable feature of GT-2 is that there is another trihelix domain at the C-terminal [11]
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