Genome-Wide Analysis of the Soybean Calmodulin-Binding Protein 60 Family and Identification of GmCBP60A-1 Responses to Drought and Salt Stresses.

Qian Yu,Jun Chen,Guo-Zhong Sun,Zhao-Shi Xu,Ming Chen,Yuan-Xia Liu,Jin-Hao Lan,Yong-Bin Zhou,You-Zhi Ma,Ya-Li Liu

doi:10.3390/ijms222413501

Abstract

Calmodulin-binding protein 60 (CBP60) members constitute a plant-specific protein family that plays an important role in plant growth and development. In the soybean genome, nineteen CBP60 members were identified and analyzed for their corresponding sequences and structures to explore their functions. Among GmCBP60A-1, which primarily locates in the cytomembrane, was significantly induced by drought and salt stresses. The overexpression of GmCBP60A-1 enhanced drought and salt tolerance in Arabidopsis, which showed better state in the germination of seeds and the root growth of seedlings. In the soybean hairy roots experiment, the overexpression of GmCBP60A-1 increased proline content, lowered water loss rate and malondialdehyde (MDA) content, all of which likely enhanced the drought and salt tolerance of soybean seedlings. Under stress conditions, drought and salt response-related genes showed significant differences in expression in hairy root soybean plants of GmCBP60A-1-overexpressing and hairy root soybean plants of RNAi. The present study identified GmCBP60A-1 as an important gene in response to salt and drought stresses based on the functional analysis of this gene and its potential underlying mechanisms in soybean stress-tolerance.

Highlights

During growth and development, plants face various biotic stresses, such as pathogen infection and herbivore attack, and abiotic stresses, including drought, heat, cold and salt [1]
In the EnsemblPlants database, 19 Calmodulin-binding protein 60 (CBP60) proteins were identified in the soybean genome
The results showed that GmCBP60A-1, GmSARD1-1 and GmSARD1-like transcripts were strongly responsive to salt stress compared with others

Summary

Introduction

Plants face various biotic stresses, such as pathogen infection and herbivore attack, and abiotic stresses, including drought, heat, cold and salt [1]. In response to abiotic stresses, various signaling pathways were activated to promote the plants sensing of the stimuli and to trigger the adequate cellular responses, which include calcium signaling pathways [2], MAPK (mitogen-activated protein kinases) cascade and other signaling pathways [3,4]. Cytoplasmic Ca2+ transduces a variety of regulatory information based on oscillations in its concentration [14,15]. This signal transmission method is called calcium oscillation. Previous studies suggest that a change of cytoplasmic Ca2+ concentration is usually induced by plant responses to a variety of biotic and abiotic stimuli [2]. Calcium ions (Ca2+) are crucial in the activation of stress-related signaling cascades [17]

Methods

Results

Conclusion