Genome-Wide Analysis of the Lateral Organ Boundaries Domain (LBD) Gene Family in Solanum tuberosum.

Hengzhi Liu,Wan Li,Chao Zhang,Minghui Ye,Peng Zhao,Nana Kong,Yunyou Nan,Yue Chen,Dongdong Wang,Chenghui Yang,Qin Chen,Lingshuang Kong,Minxuan Cao,Xiaoli Chen

doi:10.3390/ijms20215360

Abstract

Lateral organ boundaries domain (LBD) proteins belong to a particular class of transcription factors of lateral organ boundary (LOB) specific domains that play essential roles in plant growth and development. However, a potato phylogenetic analysis of the LBD family has not been fully studied by scholars and researchers. In this research, bioinformatics methods and the growth of potatoes were used to identify 43 StLBD proteins. We separated them into seven subfamilies: Ia, Ib, Ic, Id, Ie, IIa and IIb. The number of amino acids encoded by the potato LBD family ranged from 94 to 327. The theoretical isoelectric point distribution ranged from 4.16 to 9.12 Kda, and they were distributed among 10 chromosomes. The results of qRT-PCR showed that the expression levels of StLBD2-6 and StLBD3-5 were up-regulated under drought stress in the stem. The expression levels of StLBD1-5 and StLBD2-6 were down-regulated in leaves. We hypothesized that StLBD1-5 was down-regulated under drought stress, and that StLBD2-6 and StLBD3-5 up-regulation might help to maintain the normal metabolism of potato and enhance the potatoes’ resistance to drought.

Highlights

The lateral organ boundaries domain (LBD) gene refers to a gene family with a special domain of lateral organ boundaries (LOB), known as the asymmetric leaves2-like (ASL) gene family
Some genes expression levels were very high in specific tissues, such as StLBD1-5, StLBD2-6, StLBD3-5, and StLBD11-1
Induced expression analysis showed that the potato LBD gene mainly responded to immune, drought, and heat stress

Summary

Introduction

The lateral organ boundaries domain (LBD) gene refers to a gene family with a special domain of lateral organ boundaries (LOB), known as the asymmetric leaves2-like (ASL) gene family. LBD expression products represent a class of plant-specific transcription factors containing the LOB domain [2]. The LBD family has three specific protein domains which can be classified into Class I and Class II, according to their specific protein sequences, most of which belong to Class I [3,4]. They contain a similar zinc finger domain CX2CX6CX3C motif [1], a glycine-alanine-serine (GAS-block) region that it is similar to the leucine zipper-like domain, and a protein dimerization LX6LX3LX6L spiral coiled structure. Since the discovery of 43 LBD proteins in Arabidopsis, many LBD proteins have been found in plants such as

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Conclusion