Genome-Wide Analysis of the Cytochromes P450 Gene Family in Cordyceps militaris

Abstract

Cytochromes P450 (CYP450) gene family has been shown to play significant roles in various physiological processes of some species, including participate in the metabolism of various exogenous substances, synthesis of endogenous substances, abiotic and biotic stress responses and stress signaling. In this study, the members of CYP450 gene family in Cordyceps militaris (C.militaris) were identified and analyzed on the whole genome level using bioinformatics-based methods. The phylogenetic tree, gene structure, motifs and gene expression level under pH stress were analyzed. In total, 54 putative CYP450 gene family members were identified in C.militaris. The phylogenetic analysis indicated that all of the C.militaris CYP450 (CmCYP) genes, except CmCYP19, were grouped into 3 groups. The 54 CmCYP genes were randomly distributed on 7 chromosomes. The expression analysis of CmCYP under different pH environments showed the mechanism of CmCYP genes family involved in stress resistance regulation is complex and diversified. Systematic investigation of a gene family, would provide important insights into gene function and application. The results of this study will provide reference information for studying other abiotic and biotic stress response regulation mechanism of C.militaris.