Abstract
We carried out a detailed investigation of PL7 alginate lyases across the Zobellia genus. The main findings were obtained using the methods of comparative genomics and spatial structure modeling, as well as a phylogenomic approach. Initially, in order to elucidate the alginolytic potential of Zobellia, we calculated the content of polysaccharide lyase (PL) genes in each genome. The genus-specific PLs were PL1, PL6, PL7 (the most abundant), PL14, PL17, and PL40. We revealed that PL7 belongs to subfamilies 3, 5, and 6. They may be involved in local and horizontal gene transfer and gene duplication processes. Most likely, an individual evolution of PL7 genes promotes the genetic variability of the Alginate Utilization System across Zobellia. Apparently, the PL7 alginate lyases may acquire a sub-functionalization due to diversification between in-paralogs.
Highlights
Alginate Lyases in the Genus Zobellia.Marine algal polysaccharides are an important nutrient source for marine bacteria
The current studies of PULs are based on the sequencing of the genomes of cultured bacteria and metagenomes as a valuable resource of CAZymes and CAZyme machineries [4,5,6,7,8,9,10], allowing us to expand our knowledge of the bacterial degradation of algal polysaccharides
We found that Z. galactanivorans DsijT possesses the highest number of CAZymes (336), followed by Z. amurskyensis MAR 2009 138 (320), Z. uliginosa DSM 2061T (315), and Z. galactanivorans OII3 (311)
Summary
Marine algal polysaccharides are an important nutrient source for marine bacteria. To utilize these polysaccharides, which significantly differ from terrestrial plant ones, marine bacteria have developed unusual degradation mechanisms. The microorganisms feature different polysaccharide utilization loci (PULs), which encode a set of enzymes and other proteins involved in the breakdown of specific polysaccharides. The first recognized PULs were alginolytic operons associated with alginate utilization in marine Bacteroidetes [3]. The current studies of PULs are based on the sequencing of the genomes of cultured bacteria and metagenomes as a valuable resource of CAZymes and CAZyme machineries [4,5,6,7,8,9,10], allowing us to expand our knowledge of the bacterial degradation of algal polysaccharides
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