Abstract
BackgroundMung bean (Vigna radiata) is a warm-season legume crop and belongs to the papilionoid subfamily of the Fabaceae family. China is the leading producer of mung bean in the world. Mung bean has significant economic and health benefits and is a promising species with broad adaptation ability and high tolerance to environmental stresses. OSCA (hyperosmolality-gated calcium-permeable channel) gene family members play an important role in the modulation of hypertonic stress, such as drought and salinity. However, genome-wide analysis of the OSCA gene family has not been conducted in mung bean.ResultsWe identified a total of 13 OSCA genes in the mung bean genome and named them according to their homology with AtOSCAs. All the OSCAs were phylogenetically split into four clades. Phylogenetic relationship and synteny analyses showed that the VrOSCAs in mung bean and soybean shared a relatively conserved evolutionary history. In addition, three duplicated VrOSCA gene pairs were identified, and the duplicated VrOSCAs gene pairs mainly underwent purifying selection pressure during evolution. Protein domain, motif and transmembrane analyses indicated that most of the VrOSCAs shared similar structures with their homologs. The expression pattern showed that except for VrOSCA2.1, the other 12 VrOSCAs were upregulated under treatment with ABA, PEG and NaCl, among which VrOSCA1.4 showed the largest increased expression levels. The duplicated genes VrOSCA2.1/VrOSCA2.2 showed divergent expression, which might have resulted in functionalization during subsequent evolution. The expression profiles under ABA, PEG and NaCl stress revealed a functional divergence of VrOSCA genes, which agreed with the analysis of cis-acting regulatory elements in the promoter regions of VrOSCA genes.ConclusionsCollectively, the study provided a systematic analysis of the VrOSCA gene family in mung bean. Our results establish an important foundation for functional and evolutionary analysis of VrOSCAs and identify genes for further investigation of their ability to confer abiotic stress tolerance in mung bean.
Highlights
Mung bean (Vigna radiata) is a warm-season legume crop and belongs to the papilionoid subfamily of the Fabaceae family
A total of 13 VrOSCA genes were identified in mung bean and named according to the Arabidopsis orthologues (Table 1)
Phylogenetic analysis of the Hyperosmolality-gated calcium-permeable channels (OSCA) gene family in mung bean To elucidate the phylogenetic relationships of OSCA proteins in mung bean, Arabidopsis, soybean and rice, a phylogenetic tree based on the alignment of 60 fulllength OSCA protein sequences was built (Additional file 1)
Summary
Mung bean (Vigna radiata) is a warm-season legume crop and belongs to the papilionoid subfamily of the Fabaceae family. Previous studies have found that plant responses to stress mainly include the perception and transmission of signals through various pathways and the regulation of stress-responsive gene expression, resulting in physiological and morphological modifications to resist stress [6,7,8]. These changes are mainly manifested in the enhancement of proline, betaine and sugar synthesis, which helps to the maintenance of tissue water content, and the up-regulation of key antioxidant enzymes activity to reduce the oxidation of proteins and lipids by reactive oxygen species [9,10,11]. Previous studies showed that stimuli-gated Ca2+ permeable channels served as osmosensors in bacteria and animals [17, 18], which indicated that there might be specific calcium permeable channels that function as osmosensors in plants
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
