Genome-Wide Analysis of Major Facilitator Superfamily and Its Expression in Response of Poplar to Fusarium oxysporum.

Jian Diao,Wei Ma,Ping Zhang,Xiaoqian Ma,Shuxuan Li,Ling Ma,Jianyang Bai,Jiaqi Wang

doi:10.3389/fgene.2021.769888

Abstract

The major facilitator superfamily (MFS) is one of the largest known membrane transporter families. MFSs are involved in many essential functions, but studies on the MFS family in poplar have not yet been reported. Here, we identified 41 MFS genes from Populus trichocarpa (PtrMFSs). We built a phylogenetic tree, which clearly divided members of PtrMFS into six groups with specific gene structures and protein motifs/domains. The promoter regions contain various cis-acting elements involved in stress and hormone responsiveness. Genes derived from segmental duplication events are unevenly distributed in 17 poplar chromosomes. Collinearity analysis showed that PtrMFS genes are conserved and homologous to corresponding genes from four other species. Transcriptome data indicated that 40 poplar MFS genes were differentially expressed when treated with Fusarium oxysporum. Co-expression networks and gene function annotations of MFS genes showed that MFS genes tightly co-regulated and closely related in function of transmembrane transport. Taken together, we systematically analyzed structure and function of genes and proteins in the PtrMFS family. Evidence indicated that poplar MFS genes play key roles in plant development and response to a biological stressor.

Highlights

The major facilitator superfamily (MFS) is one of the largest membrane transporter families currently known (Chen et al, 2019)
We identified 41 MFS genes in P. trichocarpa
The theoretical pI of PtrMFSs is in the range of 5.54–10.07 and the aliphatic index is in the range of 85.96–118.46

Summary

Introduction

The major facilitator superfamily (MFS) is one of the largest membrane transporter families currently known (Chen et al, 2019). Utamia identifified 177 putative MFS transporters and classifified them into 17 subfamilies in Penicillium marneffei (Setyowati et al, 2020). The MFS was first characterized in 1993, when a MFS’s Response to Fusarium large class of transporters with 12 transmembrane helix domains were discovered among many membrane proteins (Marger and Saier, 1993). MFS proteins can transport many small molecules, such as monosaccharides, polysaccharides, amino acids, peptides, vitamins, enzyme cofactors, drug molecules, chromophores, and nucleotide bases (Lorca et al, 2007; Chen et al, 2008; Ming et al, 2010; Saier and Paulsen, 2011). Some MFS proteins are closely related to immunological processes, such as virus invasion and pathogenic resistance (Nicolas et al , 2005)

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Conclusion