Genome Wide Analysis and Characterization of NPR-likeGene Family of Phaseolus vulgaris L.

Abstract

Nonexpressor of pathogenesis-related genes 1 (NPR1) could be found in the regulationof pathways of salicylic acid (SA) signal transduction and systemic acquired resistance (SAR) in plants. It has been found that the members of NPR-like gene family are interconnectingwith biotic and abiotic stresses in several plants, but it has not yet been performed in common bean. In this study, in silicoanalyzes of Phaseolus vulgaris L. NPR-likegene family members were performed at the genome level. As a result of these in silico analyzes, four NPR1genes were identified and it was found that these genes have been located on four different P. vulgarischromosomes. The length of PvNPR-like proteins ranges from 475-590 amino acid residues. The theoretical isoelectric point (pI) range were found between 5.75-6.36 and all show acidic properties. As a result of phylogenetic analyzes performed between NPR-like genes of P. vulgaris, Glycine max(L.) Merr and Arabidopsis thaliana(L.) Heynh. plants, NPR-likegenes were found to be divided into three different groups (A, B and C). It has been determined that PvNPR-likegenes contain cis-actingelements that function in many biological processes, including stress and hormone responses. Differences in gene expression levels under salt and drought stress indicated that PvNPR-likegenes respond differently under stress conditions. The outcomes of this studywill supply the needed basics for understanding the function and evolutionary history of PvNPR-like genes.