Abstract
Chloroplast (ct-) and mitochondrial (mt-) DNA's from pea, spinach, bean, and lettuce leaves have been studied for their molecular size and conformation. The ct-DNA's from these plants were found to band at a density of 1.698 ± 0.001 g/cm3 and denatured with a Tm of 84° ± 0.5°C. The corresponding mt-DNA's had a buoyant density of 1.706 ± 0.001 g/cm3 and denatured with a Tm of 88° ± 0.5°C. Both ct- and mt-DNA from these plants were found to have a homogeneous melting pattern. The sheared and denatured ctand mt-DNA's from these plants were found to renature as a single kinetic class with no indication of repeating sequences. The molecular weight of the ct-DNA from the higher plants studied has been found to be 95 ± 5 x 106 by renaturation rates assuming a value of 106 x 106 for T4 DNA.
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