Abstract
Aspergillus, as a genus of filamentous fungi, has members that display a variety of different behavioural strategies, which are affected by various environmental factors. The decoded genomic sequences of many species vary greatly in their evolutionary similarities, encouraging studies on the functions and evolution of the Aspergillus genome in complex natural environments. Here, we present the 26 Mb de novo assembled high-quality reference genome of Aspergillus glaucus ‘China Changchun halophilic Aspergillus’ (CCHA), which was isolated from the surface of plants growing near a salt mine in Jilin, China, based on data from whole-genome shotgun sequencing using Illumina Solexa technology. The sequence, coupled with data from comprehensive transcriptomic survey analyses, indicated that the redox state and transmembrane transport might be critical molecular mechanisms for the adaptation of A. glaucus ‘CCHA’ to the high-salt environment of the saltern. The isolation of salt tolerance-related genes, such as CCHA-2114, and their overexpression in Escherichia coli demonstrated that A. glucus ‘CCHA’ is an excellent organism for the isolation and identification of salt tolerant-related genes. These data expand our understanding of the evolution and functions of fungal and microbial genomes, and offer multiple target genes for crop salt-tolerance improvement through genetic engineering.
Highlights
Aspergillus species exhibit amazingly diverse behavioural and physiological characteristics, as well as varied capacities to adapt to extreme environments
Strain isolation and the salt-tolerance assessment of A. glaucus ‘Changchun halophilic Aspergillus’ (CCHA)’ The halophilic fungal strain was isolated from the surface of wild vegetation growing around a saltern, and it was identified as A. glaucus strain CCHA based on morphological properties, an ITS sequence comparison (Liu et al, 2011), and its salt-tolerance level (Figs. 1A–1C)
The genome of A. glaucus ‘CCHA’, which was isolated from the surface of plants growing near a salt mine in Jilin, China, was sequenced, and the genes set of this strain were characterised and compared with those of related species
Summary
Aspergillus species exhibit amazingly diverse behavioural and physiological characteristics, as well as varied capacities to adapt to extreme environments. Measures for managing diverse environmental stresses have evolved in different Aspergillus species They are considered to be good materials for studying adaptation and responses to many. A strain of the species, A. glaucus ‘China Changchun halophilic Aspergillus’ (CCHA) was isolated from the surface of wild vegetation growing around a saltern in Jilin, China (Liu et al, 2011), which indicated that it may have a unique salt-stress resistance mechanism. Even though this strain was discovered over 10 years ago, the lack of genetic information has limited our understanding of its salt tolerance. To study the molecular mechanisms underlying the salt tolerance of A. glaucus ‘CCHA’, a high-quality genome draft was required
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